Modelling the structure of the fusion protein from human respiratory syncytial virus
Modelling the structure of the fusion protein from human respiratory syncytial virus
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DOI：
10.1093/protein/15.5.365
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发表时间：
2002-05-01
期刊：
影响因子：
--
通讯作者：
Colman, PM
中科院分区：
文献类型：
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作者：
Smith, BJ;Lawrence, MC;Colman, PM
呼吸道合胞病毒（RSV-F）的融合蛋白负责病毒颗粒与宿主细胞融合，并通过形成合胞体感染邻近细胞。 RSV-F的三维模型结构是根据新城疫病毒(NDV)中等效蛋白的结构通过同源建模得出的。尽管两种结构之间的序列同源性非常低，但该模型的大多数特征似乎具有很高的可信度，尽管 RSV-F 中的一些小区域（其二级结构预计与 NDV 中的二级结构不同）可能建模不佳。在针对单克隆抗体的逃逸突变体中鉴定出的各个残基的组织与已知的抗原位点很好地相关。还检查了几种耐药变体中涉及点突变的残基的位置。
The fusion protein of respiratory syncytial virus (RSV-F) is responsible for fusion of virion with host cells and infection of neighbouring cells through the formation of syncytia. A three-dimensional model structure of RSV-F was derived by homology modelling from the structure of the equivalent protein in Newcastle disease virus (NDV). Despite very low sequence homology between the two structures, most features of the model appear to have high credibility, although a few small regions in RSV-F whose secondary structure is predicted to be different to that in NDV are likely to be poorly modelled. The organization of individual residues identified in escape mutants against monoclonal antibodies correlates well with known antigenic sites. The location of residues involved in point mutations in several drug-resistant variants is also examined.