Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication
Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication
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Sua5p 一种单链端粒 DNA 结合蛋白，促进端粒复制
DOI：
10.1038/emboj.2009.92
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发表时间：
2009-05-20
期刊：
影响因子：
11.4
通讯作者：
Zhou, Jin-Qiu
中科院分区：
文献类型：
--
作者：
Meng, Fei-Long;Hu, Yan;Zhou, Jin-Qiu
在出芽酵母（Saccharomyces cerevisiae）中，端粒长度维持涉及一个复杂的网络，在非必需基因缺失突变体中已鉴定出超过280个端粒维持基因。作为补充，我们发现了额外的29个端粒维持基因，这些基因以前被认为是必需基因。在这项研究中，我们报道了Sua5p在端粒复制中的新功能。上位分析和端粒测序表明，sua5D细胞在早期传代时端粒逐渐缩短，Sua5在下游端粒酶募集中起作用。此外，生化、结构和遗传学研究表明，Sua5p在体外通过其表面独特的DNA结合区特异性结合单链端粒（ssTG） DNA，其DNA结合能力对其端粒功能至关重要。因此，Sua5p代表了一种新的ssTG dna结合蛋白，并在体内积极调节端粒长度。EMBO杂志（2009）28,1466-1478。doi: 10.1038 / emboj.2009.92;2009年4月16日在线发布
In budding yeast Saccharomyces cerevisiae, telomere length maintenance involves a complicated network as more than 280 telomere maintenance genes have been identified in the nonessential gene deletion mutant set. As a supplement, we identified additional 29 telomere maintenance genes, which were previously taken as essential genes. In this study, we report a novel function of Sua5p in telomere replication. Epistasis analysis and telomere sequencing show that sua5D cells display progressively shortened telomeres at early passages, and Sua5 functions downstream telomerase recruitment. Further, biochemical, structural and genetic studies show that Sua5p specifically binds single-stranded telomeric (ssTG) DNA in vitro through a distinct DNA-binding region on its surface, and the DNA-binding ability is essential for its telomere function. Thus, Sua5p represents a novel ssTG DNA-binding protein and positively regulates the telomere length in vivo. The EMBO Journal (2009) 28, 1466-1478. doi:10.1038/emboj.2009.92; Published online 16 April 2009