Chronological change and the potential of recovery on the photosynthetic efficiency of Pyropia yezoensis f. narawaensis (Bangiales) during the sporelings frozen storage treatment in the Japanese Nori cultivation
Chronological change and the potential of recovery on the photosynthetic efficiency of Pyropia yezoensis f. narawaensis (Bangiales) during the sporelings frozen storage treatment in the Japanese Nori cultivation
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DOI：
10.1111/pre.12185
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发表时间：
2017-06
影响因子：
1.5
通讯作者：
Yuki Watanabe;Tarou Morikawa;Takayuki Mine;Y. Kawamura;Gregory N. Nishihara;Ryuta Terada
中科院分区：
文献类型：
--
作者：
Yuki Watanabe;Tarou Morikawa;Takayuki Mine;Y. Kawamura;Gregory N. Nishihara;Ryuta Terada
研究了日本紫菜养殖业冷冻储存处理中培养的红藻 Pyropia yezoensis f 的光合效率随时间的变化。 narawaensis（Saga-#5 菌株，Bangiales）通过使用脉冲幅度荧光测定法。在11月育苗季后进行的干燥过程中，Nori-net上生长的配子体孢子体的最大量子产率（F v/F m）随着绝对含水量（AWC）的降低而单调下降，在20% AWC时约为0.1。在 Nori-net 冷冻储存期间，冷冻配子体的 F v/F m 较低但稳定，范围在 0.10 ± 0.02 SD 和 0.14 ± 0.05 SD 之间。冷冻处理配子体的F v/F m 大小在海水中浸泡10 min和3 h后很快恢复。浸泡10分钟和3小时后，冷冻处理14天期间这些值为0.29±0.12SD和0.47±0.05SD，冷冻处理71天后这些值为0.15±0.02SD和0.29±0.04SD，这表明恢复能力随着储存时间的增加而逐渐下降。一般栽培（即从苗圃栽培季节开始直接栽培）的F v/F m 响应与冷冻47天后的F v/F m 响应几乎相同，表明当前海苔网冷冻储存期（6或7周）对配子体并无损害。
The chronological change of photosynthetic efficiency in a frozen storage treatment of the Japanese Nori cultivation industry was examined in the cultivated red alga, Pyropia yezoensis f. narawaensis (Saga‐#5 Strain, Bangiales) by using pulse‐amplitude fluorometry. During the desiccation process that was conducted after the nursery cultivation season in November, the maximum quantum yield (F v/F m) of the gametophytic sporelings growing on the Nori‐net decreased monotonically with decreasing absolute water content (AWC), and was around 0.1 at 20% AWC. During frozen storage of the Nori‐net, the F v/F m of the frozen gametophyte was low but stable, and ranged between 0.10 ± 0.02 SD and 0.14 ± 0.05 SD. The magnitude of F v/F m for the gametophyte of the freezing treatment, after 10 min and 3 h of immersion in seawater, recovered quickly. After 10 min and 3 h of immersion, these values were 0.29 ± 0.12 SD and 0.47 ± 0.05 SD during the 14 days of freezing treatment, and 0.15 ± 0.02 SD and 0.29 ± 0.04 SD after 71 days of freezing treatment, and suggest that the ability to recover gradually decreased as the storage duration increased. The response of F v/F m from general cultivation (i.e., directly cultivated from the nursery cultivation season) and those after 47 days of freezing were almost identical, suggesting that the current Nori ‐net frozen storage period (6 or 7 weeks) was not detrimental to the gametophyte.