Efforts toward Further Integration of an Unnatural Base Pair into the Biology of a Semisynthetic Organism
Efforts toward Further Integration of an Unnatural Base Pair into the Biology of a Semisynthetic Organism
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DOI：
10.1021/jacs.1c03860
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发表时间：
2021-06-07
影响因子：
15
通讯作者：
Romesberg, Floyd E.
中科院分区：
文献类型：
--
作者：
Hashimoto, Koji;Fischer, Emil C.;Romesberg, Floyd E.
我们已经开发出半合成生物（SSO），凭借一个家庭的合成，非天然碱基对（UBP），存储和检索增加的信息。到目前为止，在SSO的转录依赖于T7噬菌体的RNA聚合酶的异源表达，在这里，我们探索将转录的内源性宿主多亚基RNA聚合酶的控制下。结果表明，E.大肠杆菌RNA聚合酶能够转录含有UBP的DNA，并且利用迄今为止鉴定的最佳UBP，应该可以选择增加非天然三磷酸的摄取。这些进展应有助于创建下一代的SSO。
We have developed semisynthetic organisms (SSOs) that by virtue of a family of synthetic, unnatural base pairs (UBPs), store and retrieve increased information. To date, transcription in the SSOs has relied on heterologous expression of the RNA polymerase from T7 bacteriophage; here, we explore placing transcription under the control of the endogenous host multisubunit RNA polymerase. The results demonstrate that the E. coli RNA polymerase is able to transcribe DNA containing a UBP and that with the most optimal UBP identified to date it should be possible to select for increased uptake of unnatural triphosphates. These advances should facilitate the creation of next generation SSOs.