Isolation and characterization of a DNA sequence complementary to an androgen-inducible messenger RNA from mouse kidney.
Isolation and characterization of a DNA sequence complementary to an androgen-inducible messenger RNA from mouse kidney.
复制标题
与小鼠肾脏雄激素诱导信使 RNA 互补的 DNA 序列的分离和表征。
DOI：
10.1016/s0021-9258(19)69091-5
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Gordon Watson
中科院分区：
文献类型：
--
作者：
Franklin;G.;Berger;Kenneth;W.;Gross;Gordon Watson
在从大小分级的含poly（A）的肾脏RNA制备的cDNA克隆库中，已鉴定出含有与小鼠肾脏睾酮诱导RNA对应的序列的克隆。采用一种新的筛选方法来特异性地检测这些序列。其中之一，pMK 908，含有一个1.2双酶对插入，进行了详细研究。RNA印迹实验表明，pMK 908插入的DNA与两个肾特异性RNA序列杂交，长度为2500和1500个核苷酸，这两个序列都是通过雌性小鼠体内睾酮处理快速诱导的。RNA过量杂交研究表明，这些RNA约占女性肾脏总RNA的0.004%，并被睾酮诱导约7-8倍。RNA已被分离并在无细胞系统中翻译，编码43，000-道尔顿多肽链。睾酮对这些RNA的诱导不依赖于脑垂体，但依赖于睾酮受体蛋白。pMK 908和其他含有睾酮诱导序列的嵌合质粒的存在，对于理解小鼠肾脏对雄激素的反应应该是有价值的。
Clones containing sequences corresponding to testosterone-inducible RNAs of mouse kidney have been identified within a cDNA clone bank prepared from size-fractionated poly(A)-containing kidney RNA. A novel screening method was employed to specifically detect such sequences. One of these, pMK908, containing a 1.2 kilobase pair insert, was studied in detail. RNA blotting experiments show that the inserted DNA of pMK908 hybridizes to two kidney-specific RNA sequences, 2500 and 1500 nucleotides in length, both of which are rapidly induced by testosterone treatment of female mice in vivo. RNA excess hybridization studies reveal that these RNAs constitute approximately 0.004% of th total RNA population in female kidneys and are induced approximately 7-8-fold by testosterone. The RNA has been isolated and translated in a cell-free system and encodes a 43,000-dalton polypeptide chain. The induction of these RNAs by testosterone is independent of the pituitary gland, but does depend upon the testosterone receptor protein. The existence of pMK908, and other chimeric plasmids containing testosterone-inducible sequences, should be valuable in understanding the response of the mouse kidney to androgens.