Highly efficient magnetic stem cell labeling with citrate-coated superparamagnetic iron oxide nanoparticles for MRI tracking
Highly efficient magnetic stem cell labeling with citrate-coated superparamagnetic iron oxide nanoparticles for MRI tracking
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DOI：
10.1016/j.biomaterials.2012.02.064
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发表时间：
2012-06-01
期刊：
影响因子：
14
通讯作者：
Ringe, Jochen
中科院分区：
文献类型：
--
作者：
Andreas, Kristin;Georgieva, Radostina;Ringe, Jochen
跟踪移植的干细胞对于监测基于细胞的治疗的安全性和有效性至关重要。磁共振成像（MRI）提供了一个非常敏感的，重复的和非侵入性的体内检测磁标记的细胞，但标记与商业超顺磁性氧化铁纳米粒子（SPION）仍然是有问题的，因为低标记效率和需要潜在的毒性转染剂。在这项研究中，新的实验柠檬酸盐涂层SPION和商业Endorem和Resovist SPION进行了比较，在体外标记效率，对干细胞功能和体内MRI可视化的影响。在没有转染剂的情况下，仅使用柠檬酸盐SPION实现了人间充质干细胞（MSC）的有效标记。磁性标记的人骨髓间充质干细胞不影响细胞增殖，典型的细胞表面标志物抗原的介绍和分化成脂肪和成骨谱系。然而，软骨分化和趋化性显着受损增加SPION掺入。肌内注射SPION标记的骨髓间充质干细胞后，通过7 T-MRI在体内观察，并通过普鲁士蓝和免疫组化染色在体外回收。尽管SPION掺入、细胞功能和MRI可视化的仔细滴定是必不可少的，但柠檬酸盐SPION是通过MRI进行体内干细胞追踪的非常有效的细胞内磁性标记。(C)2012爱思唯尔有限公司保留所有权利。
Tracking of transplanted stem cells is essential to monitor safety and efficiency of cell-based therapies. Magnetic resonance imaging (MRI) offers a very sensitive, repetitive and non-invasive in vivo detection of magnetically labeled cells but labeling with commercial superparamagnetic iron oxide nanoparticles (SPIONs) is still problematic because of low labeling efficiencies and the need of potentially toxic transfection agents. In this study, new experimental citrate-coated SPIONs and commercial Endorem and Resovist SPIONs were investigated comparatively in terms of in vitro labeling efficiency, effects on stem cell functionality and in vivo MRI visualization. Efficient labeling of human mesenchymal stem cells (MSCs) without transfection agents was only achieved with Citrate SPIONs. Magnetic labeling of human MSCs did not affect cell proliferation, presentation of typical cell surface marker antigens and differentiation into the adipogenic and osteogenic lineages. However, chondrogenic differentiation and chemotaxis were significantly impaired with increasing SPION incorporation. Transplanted SPION-labeled MSCs were visualized in vivo after intramuscular injection in rats by 7T-MRI and were retrieved ex vivo by Prussian Blue and immunohistochemical stainings. Though a careful titration of SPION incorporation, cellular function and MRI visualization is essential, Citrate SPIONs are very efficient intracellular magnetic labels for in vivo stem cell tracking by MRI. (C) 2012 Elsevier Ltd. All rights reserved.