Excitation of lateral vestibular neurons by peripheral afferent fibers.
Excitation of lateral vestibular neurons by peripheral afferent fibers.
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外周传入纤维刺激外侧前庭神经元。
DOI：
10.1152/jn.1966.29.3.508
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发表时间：
1966
影响因子：
2.5
通讯作者：
B. Peterson
中科院分区：
文献类型：
--
作者：
V. J. Wilson;M. Kato;R. Thomas;B. Peterson
方法所有实验均在猫身上进行。大多数动物用40 mg/kg氯醛糖-尿素(40 mg/kg)腹腔注射麻醉。氯醛糖和800 mg/kg。溶于水中，或在最近的实验中，溶于聚乙二醇中。两只猫用戊巴比妥钠麻醉(首次注射40 mg/kg)。腹膜腔内注射，然后静脉补充)。所有手术程序完成后，动物通过静脉注射三硫氰胺(Flaxdil，美国氰胺公司)固定。进行人工呼吸。在所有的实验中，腰椎椎板切除，从L1到SZ，以通常的方式进行，在最近的实验中，也进行了较小范围的颈椎椎板切除，从大约C，到C5，也被执行。在放置刺激电极后(见下文)，腰髓被一池温暖的矿物油覆盖，而颈髓被棉花和海绵覆盖，两者都用林格溶液润湿。解剖了许多周围神经，并准备进行刺激。暴露于同侧后肢的神经有腓肠肌(SuR)、腓肠肌-比目鱼肌(GS)、足底(PLANT)、腓总神经(CP)和腿筋神经(HS)，而CP有时又分为腓浅肌(SP)和胫骨前伸肌(TA+EL)。在对侧后肢，通常准备HS、SUR、CP和PLANT。同侧前肢浅神经也常被解剖。在最初的实验中，猫被放在一个立体定位头架上，暴露并移除天幕，然后通过吸力移除小脑，暴露出第四脑室的底部。有了这样的准备，就有可能搜索电极在垂直Horsley-Clarke平面上定向的细胞，然后在同一平面上切割组织切片。在最近的实验中，头部弯曲，天幕完好无损，只有小脑被移除；这些动物的组织移除范围较小，这导致了更好的实验准备。在实验中，暴露的脑干被一池林格溶液覆盖，该溶液被保持在接近体温的温度。这个储液池是从一个上升的储液池中不断补充的，在这个储液池中，液体与95Y0和5Y0CO的混合气体保持平衡；通过稳定的吸力，储液池的水位保持大致恒定。在一些实验中，呼吸或血管搏动引起的运动干扰了动作电位的记录。通常先进行双侧气胸检查。
METHODSAll experiments were performed on cats. Most animals were anesthetized by intraperitoneal injections of chloralose-urethan(40 mg/kg. chloralose and 800 mg/kg. urethan) dissolved in water or, in the most recent experiments, in polyethylene glycol (mol wt ZOO). Two cats were anesthetized with pentobarbital(initial injection 40 mg/kg. intraperitoneally, followed by intravenous supplements). After completion of all operative procedures the animals were immobilized by intravenous injections of gallamine triethiodide(Flaxedil, American Cyanamid Co.) and artificially respired. In all experiments a lumbar laminectomy, from L1 to SZ, was performed in the usual manner, and in the more recent experiments a less extensive cervical laminectomy, from approximately C, to C5, was also performed. After placement of stimulating electrodes(see below) the lumbar cord was covered by a pool of warm mineral oil while the cervical cord was covered by cotton and sponges, both moistened with Ringer solution. Many peripheral nerves were dissected and prepared for stimulation. The nerves exposed in the ipsilateral hind limb were sural(SUR), gastrocnemius-soleus(GS), plantaris(PL), flexor longus digitorum plus tibialis posterior(FDL), plantar(PLANT), common peroneal(CP) and hamstring(HS); in some experiments HS was further dissected to biceps posterior-semitendinosus(BST), biceps anterior(BA), and semimembranosus(SM), while CP was at times divided into superficial peroneal(SP) and tibialis anterior plus extensor longus digitorum (TA+ EL). In the contralateral hind limb HS, SUR, CP, and PLANT were usually prepared. The superficial radial nerve of the ipsilateral forelimb was also usually dissected. In the initial experiments cats were placed in a stereotaxic head holder, the tentorium was exposed and removed, and the cerebellum then removed by suction, exposing the floor of the fourth ventricle. With such preparations it was possible to search for cells with electrodes oriented in the vertical Horsley-Clarke plane and subsequently to cut histological sections in the same plane. In the more recent experiments the head was flexed, the tentorium left intact, and only the cerebellum removed; the removal of tissue in these animals was less extensive, and this resulted in better experimental preparations. During the experiment the exposed brain stem was covered by a pool of Ringer solution that was kept at a temperature close to body temperature. This pool was continually replenished from a raised reservoir in which the fluid was equilibrated with a gas mixture of 95 y0 0, and 5 y0 CO,; the level of the pool was kept approximately constant by steady suction. In some experiments movements due to respiration or to pulsation of ‘blood vessels interfered with recording of action potentials. Usually a bilateral pneumothorax was performed as a first