Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates.
Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates.
复制标题
DOI：
10.1093/clinchem/34.11.2357
复制
发表时间：
1988-11
影响因子：
9.3
通讯作者：
D W Horne;D. Patterson
中科院分区：
文献类型：
--
作者：
D W Horne;D. Patterson
微生物测定法仍广泛用于血清和其他生物样品中叶酸衍生物的测定。我们在这里描述了一种涉及使用96孔微滴板的程序修改。该程序与现代计算机接口的微量滴度板读取器和数据还原软件一起使用，大大缩短了该测定的时间并最大限度地降低了试剂成本。在我们的检测程序条件下，所有叶酸衍生物对干酪乳杆菌有相同的生长反应。大鼠肝脏提取物的测定结果表明，标准生物测定法与96孔法非常吻合。
Microbiological assay is still widely used for estimating folic acid derivatives in serum and other biological samples. We describe here a modification of this procedure involving use of 96-well microtiter plates. This procedure, used with modern, computer-interfaced microtiter-plate readers and data-reduction software, greatly shortens the time and minimizes reagent costs for this assay. Under the conditions of our assay procedures, all folic acid derivatives tested gave equal growth response for Lactobacillus casei. Results for assays of rat liver extracts showed excellent agreement between the standard bioassay and the 96-well procedure.