Development of biochemical reaction unit by using magnetic droplet-handling mechanism
Development of biochemical reaction unit by using magnetic droplet-handling mechanism
复制标题
利用磁性液滴处理机制开发生化反应装置
DOI：
10.1109/sensor.2005.1497381
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
K. Sato
中科院分区：
文献类型：
--
作者：
K. Takayanagi;M. Shikida;H. Honda;H. Ito;R. Kato;K. Sato
我们之前提出了一种用于微全分析系统(Micro-TAS)的磁性液滴处理装置，并确定了其工作原理。该设备的优点是不需要泵和阀门等机械设备来处理解决方案。此后，通过应用液滴处理机制，我们进一步开发了生化反应单元芯片，这是我们微型TAS中的关键部件。我们证实，利用碱性磷酸酶催化的对-硝基苯基磷酸酯的水解，我们可以在不损失酶活性的情况下进行酶反应。我们还证实了所获得的酶免疫测定的校准曲线在酶反应中是线性的。在反应芯片上获得了70%的反应效率。结果表明，所研制的反应芯片适用于酶免疫检测系统。
We previously proposed a magnetic droplet-handling device for a micro-total analysis system (micro-TAS) and confirmed its operation principle. The device has the advantage of not requiring mechanical devices, such as pumps and valves, for handling solutions. Since then, we have further developed the biochemical reaction unit chip, which is a key component in our micro-TAS, by applying the droplet-handling mechanism. We confirmed that we could perform an enzymatic reaction without losing the enzymatic activity, by utilizing the alkaline phosphatase-catalyzed hydrolysis of p-nitrophenyl phosphate. We also confirmed that the obtained calibration curves for an enzyme immuno assay had linearity in the enzymatic reaction. We obtained a reaction efficiency of 70% on the reaction chip. From these results, we concluded that the developed reaction chip is applicable in enzyme immuno assay systems.