Structure and expression of the Drosophila calmodulin gene.
Structure and expression of the Drosophila calmodulin gene.
复制标题
果蝇钙调蛋白基因的结构和表达。
DOI：
10.1093/nar/15.8.3335
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发表时间：
1987
影响因子：
14.9
通讯作者：
Tobin,SL
中科院分区：
文献类型：
--
作者：
Yamanaka,MK;Saugstad,JA;Hanson-Painton,O;McCarthy,BJ;Tobin,SL
我们分离并鉴定了代表黑腹果蝇钙调素基因的cDNA和基因组克隆。通过基因组印迹和重建实验证明，钙调蛋白基因在果蝇基因组中只存在一次。将克隆探针与三龄幼虫的多烯染色体杂交，使该基因定位在第二染色体左臂49A区。该基因产生了两个1.65 kb和1.9 kb的转录本。在果蝇发育的几个阶段测量了钙调素信息的积累。这些实验的结果表明基因的发育调控。三个中间序列中断了蛋白质编码核苷酸，其中两个位于钙调蛋白功能域内。给出了编码该蛋白的DNA序列；所得氨基酸序列与其他物种的氨基酸序列进行了比较。讨论了果蝇钙调素基因与其他物种的钙调素基因以及与其他钙结合蛋白基因的结构相似性。
We have isolated and characterized cDNA and genomic clones representing the calmodulin gene ofDrosophila melanogaster. As demonstrated by genomic blots and by reconstruction experiments, the calmodulin gene is represented once in theDrosophilagenome.In situhybridization of cloned probes to the polytene chromosomes of third instar larvae permitted the localization of the gene to region 49A on the left arm of the second chromosome. Two transcripts of 1.65 and 1.9 kb are produced from this gene. The accumulation of calmodulin message was measured at several stages ofDrosophiladevelopment. The results of these experiments suggest developmental regulation of the gene. Three intervening sequences interrupt the protein coding nucleotides and two of these are located within calmodulin functional domains. The DNA sequence encoding the protein is presented; the derived amino acid sequence is compared to that of other species. The structural similarities of theDrosophilacalmodulin gene to calmodulin genes of other species and to other calcium binding protein genes are discussed.
DOI：
10.1016/0006-291x(66)90447-5
发表时间：
1966-01-01
影响因子：
3.1
作者：
DENHARDT, DT
通讯作者：
DENHARDT, DT