Simultaneous measurement of dopamine and its metabolites, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid and tryptophan in brain tissue using liquid chromatography and electrochemical detection.
Simultaneous measurement of dopamine and its metabolites, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid and tryptophan in brain tissue using liquid chromatography and electrochemical detection.
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采用液相色谱和电化学检测技术同时测定脑组织中的多巴胺及其代谢物5-羟色胺、5-羟基吲哚乙酸和色氨酸。
DOI：
10.1016/0024-3205(82)90004-2
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发表时间：
1982
期刊：
影响因子：
6.1
通讯作者：
Lyness,WH
中科院分区：
文献类型：
--
作者：
Lyness,WH
建立了一种用反相液相色谱电化学检测法测定脑色氨酸的方法。该方法同时测定多巴胺（DA）及其代谢产物，二羟基苯乙酸（DOPAC）和高香草酸（HVA），以及5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）。该方法不需要从离子交换树脂中洗脱。脱蛋白和离心后，将样品直接进样至色谱柱上。结果发现，移动的相pH值的微小变化显着影响色氨酸的保留时间，而吲哚胺和儿茶酚胺的洗脱没有变化。这些内源性化合物在单次注射中的测定证明不仅方便而且便宜。使用氧化铝和离子交换树脂获得的值产生相当的值。
An assay has been developed for brain tryptophan using reverse-phase liquid chromatography with electrochemical detection. The method simultaneously assays dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), as well as 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA). The method does not require elution from ion exchange resins. After deproteinization and centrifugation samples are injected directly onto the chromatographic column. It was found that small changes in mobile phase pH markedly influenced the retention time of tryptophan while elution of the indoleamines and catecholamines did not change. The assay of these endogenous compounds in a single injection proved not only expedient but inexpensive. Values obtained using alumina and ion exchange resins yielded comparable values.