Distribution of antinociceptive adenosine a, receptors in the spinal cord dorsal horn, and relationship to primary afferents and neuronal subpopulations
Distribution of antinociceptive adenosine a, receptors in the spinal cord dorsal horn, and relationship to primary afferents and neuronal subpopulations
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DOI：
10.1016/s0306-4522(03)00480-9
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Molander, C
中科院分区：
文献类型：
--
作者：
Schulte, G;Robertson, B;Molander, C
腺苷可以减轻动物和人类的疼痛和痛觉异常，这可能是通过脊髓腺苷A受体实现的。本研究用免疫组织化学、原位杂交、放射配基结合和共聚焦显微镜技术研究了腺苷A受体在大鼠脊髓背角的分布。在腰髓背角，II板层内侧有密集的免疫反应阳性反应，但不受背根切断或胸髓半横断的影响。共聚焦显微镜显示背角有密切的解剖关系，但A受体之间没有或仅有少量重叠，与初级传入中枢终末相关的标记物：降钙素基因相关肽或Isoectin B4，或与神经元亚群：Mu-阿片受体，神经元型一氧化氮合酶，甲硫氨酸脑啡肽，小白蛋白，或蛋白激酶CGamma，或与胶质细胞：胶质纤维酸性蛋白。少数腺苷A受体阳性结构被alpha-amino-3-hydroxy-5-methyl-4-isoaxolepropionic酸性谷氨酸受体亚基1和2/3双标。结果表明，背角内的腺苷A受体大部分位于突触后内层11神经元胞体和突起，其功能和神经化学特征尚不清楚。许多腺苷A受体阳性结构与异凝素134阳性C纤维初级传入和/或突触后结构密切接触，这些结构含有对伤害性信息调制具有重要作用的成分。(C)2003年IBRO。爱思唯尔有限公司出版。保留所有权利。
Adenosine can reduce pain and allodynia in animals and man, probably via spinal adenosine A, receptors. In the present study, we investigate the distribution of the adenosine A, receptor in the rat spinal cord dorsal horn using immunohistochemistry, in situ hybridization, radioligand binding, and confocal microscopy. In the lumbar cord dorsal horn, dense immunoreactivity was seen in the inner part of lamina II. This was unaltered by dorsal root section or thoracic cord hemisection. Confocal microscopy of the dorsal horn revealed close anatomical relationships but no or only minor overlap between A, receptors and immunoreactivity for markers associated with primary afferent central endings: calcitonin gene-related peptide, or isolectin B4, or with neuronal subpopulations: mu-opioid receptor, neuronal nitric oxide synthase, met-enkephalin, parvalbumin, or protein kinase Cgamma, or with glial cells: glial fibrillary acidic protein. A few adenosine A, receptor positive structures were double-labeled with alpha-amino-3-hydroxy-5-methyl-4-isoaxolepropionic acid glutamate receptor subunits 1 and 2/3. The results indicate that most of the adenosine A, receptors in the dorsal horn are located in inner lamina 11 postsynaptic neuronal cell bodies and processes whose functional and neurochemical identity is so far unknown. Many adenosine A, receptor positive structures are in close contact with isolectin 134 positive C-fiber primary afferents and/or postsynaptic structures containing components of importance for the modulation of nociceptive information. (C) 2003 IBRO. Published by Elsevier Ltd. All rights reserved.