Intraspecific sequence variation of chloroplast DNA in Pedicularis chamissonis Steven (Scrophulariaceae) and geographic structuring of the Japanese ''Alpine'' plants
Intraspecific sequence variation of chloroplast DNA in Pedicularis chamissonis Steven (Scrophulariaceae) and geographic structuring of the Japanese ''Alpine'' plants
复制标题
DOI：
10.1007/bf02509308
复制
发表时间：
1997-06-01
影响因子：
2.8
通讯作者：
Shimizu, T
中科院分区：
文献类型：
--
作者：
Fujii, N;Ueda, K;Shimizu, T
为了弄清茶条马尾藻种内多样性的进化模式和进化过程，我们分析了叶绿体DNA非编码区核苷酸序列的种内变异：在24个茶条马尾藻种群中，TrnT(UGu)与trnL(Uaa)5‘外显子之间、trnL(Uaa)3’外显子与trnF(Gaa)之间、atpB与rbcL之间存在33个核苷酸替换和12个插入/缺失，它们的遗传距离在0.001~0.014之间，可识别出17种不同的cpDNA单倍型，每种单倍型都具有地理结构。在cpDNA单倍型的系统发育分析中发现了两个主要分支(北方和南方分支)。北方支系的单倍型在日本本州中部、阿留申群岛的乌纳拉斯卡岛以北的MTS种群中分布范围较广。大多数单倍型之间的关系是未解决的多倍体。另一方面，南方支系的单倍型分布从加山山种群向南至本州中部荒川山种群。在这个分支中，我们清楚地识别出三个亚支，根据这些结果，我们得出结论，沙米索尼群岛北部和南部分支的单倍型可能在没有一个冰期从北部向下迁移到日本群岛。
In order to clarify evolutionary patterns and processes of intraspecific diversification of Pedicularis chamissonis Steven, we analyzed intraspecific variation of the nucleotide sequences of non-coding regions of chloroplast DNA: the intergenic spacers between trnT (UGU) and trnL (UAA) 5'exon, trnL (UAA) 3'exon and trnF (GAA), and atpB and rbcL, In 24 populations of P. chamissonis, 33 nucleotide substitutions and 12 insertions/deletions were inferred, and their genetic distances ranged from 0.001 to 0.014, Seventeen distinct cpDNA haplotypes could be recognized and each haplotype was found to be geographically structured. Two major clades (the Northern and Southern clades) were revealed in phylogenetic analyses of cpDNA haplotypes. The haplotypes of the Northern clade had a wider distribution area in the populations of Mts, Iide of central Honshu in Japan, northward to Unalaska Island in the Aleutians. Relationships among most haplotypes were unresolved polytomies. On the other hand, the haplotypes of the Southern clade occurred from the populations of Mt, Gassan southwards to Mt, Arakawa of central Honshu. Within this clade, three subclades were clearly recognized, From these results, we concluded that the haplotypes of the Northern and Southern clades in P. chamissonis might have traveled down to Japanese Archipelago from the north in not a single glacial period.