Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma.
Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma.
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发表时间：
1995-07
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通讯作者：
G. Bolelli;P. Muti;A. Micheli;R. Sciajno;F. Franceschetti;V. Krogh;P. Pisani;F. Berrino
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作者：
G. Bolelli;P. Muti;A. Micheli;R. Sciajno;F. Franceschetti;V. Krogh;P. Pisani;F. Berrino
基于低温储存生物样本的前瞻性研究为癌症病因学研究开辟了新的视角。在计划这些研究时，一个关键问题是评估样本的长期保存是否能够影响所涉及主题的分类。 ORDET 项目是一项关于乳腺癌病因学中激素和饮食的前瞻性研究，提供了 -80 摄氏度的生物库，在该项目的框架内，我们评估了 3 年冷冻保存期间血清和血浆样本中雌二醇、游离睾酮和总睾酮以及催乳素的稳定性。研究结果表明，在整个三年期间，受试者所有激素的激素浓度几乎保持相同的排名。从随时间的稳定性来看，雌二醇、催乳素和总睾酮在血清和血浆中都具有相当好的性能。无血清睾酮随着时间的推移增加了 30%，而孕酮则减少了初始浓度的约 40%。然而，按激素水平进行个体分类的可靠性表明低温储存对于流行病学目的的有效性，至少对于激素参数而言。
Prospective studies based on the storage of biological samples at low temperature have opened new perspectives in etiological research on cancer. In planning these studies a crucial question is to evaluate whether the long-term preservation of samples is able to affect the categorization of the subjects involved. In the frame of the ORDET project, a prospective study of hormones and diet in the etiology of breast cancer provided with a -80 degrees C biological bank, we have evaluated the stability of estradiol, free and total testosterone, and prolactin in serum and plasma samples over 3 years of cryoconservation. Study results showed that the subjects maintained almost the same rank by hormonal concentration throughout the 3-year period for all hormones. Looking at the stability over time, estradiol, prolactin, and total testosterone had fairly good performance for both serum and plasma. Serum-free testosterone increased in time up to 30%, whereas progesterone decreased by about 40% of the initial concentration. However, the reliability of the individual categorization by hormonal level suggests the validity of low temperature storage for epidemiological purposes, at least for hormonal parameters.