Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity
Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity
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DOI：
10.1016/j.fbp.2020.08.005
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发表时间：
2020-11-01
影响因子：
4.6
通讯作者：
Agrawal, Deepti
中科院分区：
文献类型：
--
作者：
Baral, Pratibha;Pundir, Anushka;Agrawal, Deepti
由于廉价的剩余能源选择以及通过热电联产获得的激励较低，甘蔗渣（SCB）预计会成为印度废物管理的一个潜在威胁。通过生物技术干预，高效利用甘蔗渣被视为一个机遇。本研究旨在从甘蔗渣中快速生产富含葡萄糖的浓缩水解产物。选择碱预处理的生物质，使用新一代纤维素酶混合物Cellic CTec2（按葡聚糖含量25毫克/克投加）进行水解。采用两步（9% + 9%）底物添加策略，间隔一小时，并以三种不同方式终止糖化。无论采用何种终止方法，约84.5%的纤维素被水解，从18%的生物质中释放出≥100克/升的葡萄糖。使用嗜热凝结芽孢杆菌NCIM 5648，直接使用富含葡萄糖的滤液可产生69.2 ± 2.5克/升的L（+）乳酸。在本研究的分步水解和发酵（SHF）过程中，获得的最佳葡萄糖和乳酸生产率分别为5.27克/升·小时和2.88克/升·小时。展示了一种绿色且可持续的工艺，用于以高生产率从甘蔗渣生产具有工业相关性的糖类，并将其转化为生物基乳酸。（C）2020化学工程师学会。由爱思唯尔有限公司出版。保留所有权利。
Sugarcane bagasse (SCB) is anticipated to emerge as a potential threat to waste management in India on account of cheap surplus energy options and lower incentives through its co-generation. Through biotechnological intervention, the efficient utilization of SCB is seen as an opportunity. The present study aimed towards expeditious production of concentrated glucose-rich hydrolysate from SCB. Alkali pretreated biomass was chosen for hydrolysis with a new generation cellulase cocktail, Cellic CTec2 dosed at 25 mg g-1 glucan content. A two-step (9% + 9%) substrate feeding strategy was adopted with a gap of an hour, and saccharification was terminated in three different ways. Irrespective of the methods employed for termination, similar to 84.5% cellulose was hydrolyzed releasing >= 100 g L-1 glucose from 18% biomass. Direct use of glucose-rich filtrates yielded 69.2 +/- 2.5 g L-1 of L (+) lactic acid using thermophilic Bacillus coagulans NCIM 5648. The best-attained glucose and lactic acid productivities during separate hydrolysis and fermentation (SHF) in the present study were 5.27 and 2.88 g L-1 h(-1), respectively. A green and sustainable process is demonstrated for the production of industrially relevant sugars from SCB at high productivity and its valorization to bio-based lactic acid. (C) 2020 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.