UPLC-QqQ-MS/MS-based phenolic quantification and antioxidant activity assessment for thinned young kiwifruits
UPLC-QqQ-MS/MS-based phenolic quantification and antioxidant activity assessment for thinned young kiwifruits
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DOI：
10.1016/j.foodchem.2018.12.062
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发表时间：
2019-05-30
期刊：
影响因子：
8.8
通讯作者：
Quek, Siew Young
中科院分区：
文献类型：
--
作者：
Jiao, Yang;Chen, Donglin;Quek, Siew Young
本文首次对猕猴桃幼果作为废弃农业资源进行了研究，并对其酚类特性和抗氧化能力进行了评价。两批不同成熟期的TYK （TYK1和TYK2）和成熟的猕猴桃（MK）来自三个新西兰品种。与MK相比，TYK，特别是TYK1的总酚含量（7.92倍）、总黄酮含量（17.56倍）和总黄酮醇含量（10.11倍）显著提高，抗氧化能力显著提高，且与酚相关。建立了一种低检出限（0.010 ~ 0.240 mg/L）、高精度（rsd < 6.79%）的uplc - qq -MS/MS方法，用于定量猕猴桃提取物中15种酚类物质。表儿茶素是TKY和MK的主要抗氧化剂，其中Zesy002品种TYK1的FDW含量最高，为2989.23 μ g/g。这些结果为这些品种的TYK在食品和保健品中的开发利用提供了良好的依据。
For the first time, thinned young kiwifruits (TYK) have been investigated as a source of deserted agriculture resource and evaluated for their phenolic characteristics along with antioxidant capacity. Two batches of TYK in different maturity stages (TYK1 and TYK2) and mature kiwifruits (MK) were sourced from three New Zealand cultivars. Comparing with MK, the TYK, especially TYK1, exhibited significantly higher contents of total phenolics (7.92 fold), total flavonoids (17.56 fold) and total flavanols (10.11 fold) as well as elevated and phenoliccorrelated antioxidant capacities. A novel UPLC-QqQ-MS/MS method with low limit of detection (0.010-0.240 mg/L) and high accuracy (RSDs < 6.79%) was established to quantify 15 phenolics in the kiwifruit extracts. Epicatechin was the prevailing antioxidant in both TKY and MK, with the highest level of 2989.23 mu g/g FDW present in TYK1 of 'Zesy002' cultivar. These results provide good evidence for the development and utilization of TYK of these cultivars in food and nutraceuticals.