A Novel Near-Infrared Fluorescent Probe TMTP1-PEG4-ICG for in Vivo Tumor Imaging
A Novel Near-Infrared Fluorescent Probe TMTP1-PEG4-ICG for in Vivo Tumor Imaging
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用于体内肿瘤成像的新型近红外荧光探针 TMTP1-PEG4-ICG
DOI：
10.1021/acs.bioconjchem.8b00756
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发表时间：
2018
影响因子：
4.7
通讯作者：
Ling Xi
中科院分区：
文献类型：
--
作者：
Ying zhou;Guiying Jiang;Wang Wei;Rui Wei;Xi Chen;Xueqian Wang;Juncheng Wei;Ding Ma;Fei Li;Ling Xi
分子显像剂被认为是。有前途的示踪剂肿瘤成像和指导治疗。通过FliTrx细菌肽筛选TMTP1。显示系统在我们的实验室之前，并显示给。特异性针对原发肿瘤和转移灶。在。本研究设计小肽TMTP1偶联。为近红外荧光剂ICG的衍生物ICG- osu。通过PEG4，形成新型探针TMTP1-PEG4-ICG。成功合成并通过CCK-8法鉴定。表明它对正常细胞和癌细胞无毒。动力学研究表明探针通过肝-肠和肾-膀胱途径被清除。肿瘤。利用流式细胞术对4T1、SiHa、HeLa、S12和HaCaT细胞进行体外靶向能力评价。体内。4T1和HeLa荷瘤小鼠的显像进一步证实了肿瘤归巢能力。正如我们所料，探测器。无论对小鼠肿瘤还是人源化肿瘤，在体内和体外均表现出对肿瘤细胞极好的亲和力。在。结论TMTP1-PEG4-ICG在荷瘤小鼠模型中具有理想的显像效果，为其提供了新的研究机会。用于肿瘤诊断或指导切除。
Molecular imaging agents are considered to be.promising tracers for tumor imaging and guided therapy..TMTP1 was screened through the FliTrx bacterial peptide.display system in our laboratory previously and shown to.speci fi cally target to primary tumors and metastatic foci. In.this study, small peptide TMTP1 was designed to conjugate.to a near-infrared fl uorescent agent ICG derivative ICG-OSu.through PEG4, forming the novel probe TMTP1-PEG4-ICG..It was successfully synthesized and certi fi ed. CCK-8 assay.showed that it was nontoxic to normal cells and cancerous.cells. Dynamics study indicated that the probe was cleared through the liver − intestine and kidney − bladder pathway. Tumor.targeting capability of this probe in vitro was evaluated on 4T1, SiHa, HeLa, S12, and HaCaT cells by fl ow cytometry. In vivo.imaging of 4T1 and HeLa tumor-bearing mice further identi fi ed the tumor homing ability. As we had expected, the probe.showed excellent a ffi nity to cancer cells not only in vitro but also in vivo, whether in murine tumor or humanized tumor. In.conclusion, TMTP1-PEG4-ICG demonstrated ideal imaging e ff ects on tumor-bearing mice model, providing new opportunities.for tumor diagnostic or guiding resection.