Effect of Borrelia burgdorferi OspC at the Site of Inoculation in Mouse Skin
Effect of Borrelia burgdorferi OspC at the Site of Inoculation in Mouse Skin
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DOI：
10.1128/iai.00464-10
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发表时间：
2010-11-01
影响因子：
3.1
通讯作者：
Coburn, Jenifer
中科院分区：
文献类型：
--
作者：
Antonara, Styliani;Ristow, Laura;Coburn, Jenifer
伯氏疏螺旋体表面脂蛋白OspC是一个关键的毒力因子，但其在伯氏疏螺旋体感染建立中的确切作用尚不清楚。为了确定OspC是否会影响接种部位的宿主反应，在感染部位检测了野生型、OspC突变型和补充突变型伯氏疏螺旋体菌株的巨噬细胞和中性粒细胞的募集以及细胞因子的产生。在21种细胞因子中，单核细胞趋化蛋白1 （MCP-1）、角化细胞来源的趋化因子（KC, CXCL1）和血管内皮生长因子（VEGF）在接种伯氏疏螺旋体时水平升高，并且在感染1周的过程中，在一个或多个时间点随着OspC的产生而变化。不同细胞因子对伯氏疏螺旋体OspC的表达动力学和依赖性存在差异。KC和MCP-1的产生以及单核细胞浸润的出现与细菌的存在相关，而不是与OspC特异性相关。相反，VEGF的产生并不仅仅与细菌的存在相关，而是受到OspC存在的影响。在体外实验中，OspC和表达OspC的伯氏疏螺旋体比对照组更能刺激内皮细胞的生长。这些数据表明OspC可能在其哺乳动物和蜱宿主的界面上在伯氏疏螺旋体的生命中起着新的作用。
The Borrelia burgdorferi surface lipoprotein OspC is a critical virulence factor, but its precise role in the establishment of B. burgdorferi infection remains unclear. To determine whether OspC affects the host response at the site of inoculation of the bacterium, the recruitment of macrophages and neutrophils and the production of cytokines were examined at the site of infection by wild-type, ospC mutant, and complemented mutant B. burgdorferi strains. Of the 21 cytokines tested, monocyte chemoattractant protein 1 (MCP-1), keratinocyte-derived chemokine (KC, CXCL1), and vascular endothelial growth factor (VEGF) were found at increased levels at the site of inoculation of B. burgdorferi, and the levels varied with the production of OspC at one or more time points over the 1-week course of infection. The kinetics of expression and the dependence on OspC production by B. burgdorferi varied among the cytokines. The production of KC and MCP-1, and the appearance of monocytic infiltrates, correlated with the presence of the bacteria rather than with OspC specifically. In contrast, VEGF production was not correlated simply to the presence of the bacteria and is influenced by the presence of OspC. In in vitro assays, OspC and B. burgdorferi expressing OspC stimulated the growth of endothelial cells more than did the controls. These data suggest the possibility of a novel role for OspC in the life of B. burgdorferi at the interface of its mammalian and tick hosts.