Patternable protein resistant surfaces for multifunctional microfluidic devices via surface hydrophilization of porous polymer monoliths using photografting
Patternable protein resistant surfaces for multifunctional microfluidic devices via surface hydrophilization of porous polymer monoliths using photografting
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DOI：
10.1021/cm0617034
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发表时间：
2006-12-12
影响因子：
8.6
通讯作者：
Frechet, Jean M. J.
中科院分区：
文献类型：
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作者：
Stachowiak, Timothy B.;Svec, Frantisek;Frechet, Jean M. J.
利用亲水性单体的单步和两步光接枝制备了抗蛋白质吸附的表面改性大孔聚合物单体。用荧光素标记的牛血清白蛋白荧光法测定蛋白质的吸附。接枝丙烯酰胺、甲基丙烯酸2-羟乙酯、乙烯基吡咯烷酮和聚甲基丙烯酸乙二醇酯（PEGMA）单体，并评价其防止蛋白质吸附的能力。与未修饰的表面相比，光接枝的PEGMA层将蛋白质吸附降低到不到2%。连续的两步光接枝过程包括：(i)形成共价结合的表面光引发剂位点，然后（ii）表面局部接枝聚合。光接枝过程中单体浓度和辐照时间是优化两步工艺的最重要参数。对于同时进行的单步光接枝，溶剂和光引发剂的存在是关键因素。无引发剂单步光接枝技术的效率低于两步技术，但在接枝后较长时间内对蛋白质吸附的预防效果相似。光接枝作为一种简单的、可模式化的修饰技术的应用被证明了，首先在单体内创建一个亲水表面，随后用一层活性的2-乙烯基-4,4-二甲基内酯聚合物链进行模式化，然后固定化绿色荧光蛋白。
Surface-modified macroporous polymer monoliths that resist the adsorption of proteins have been prepared using both single- and two-step photografting of hydrophilic monomers. The adsorption of protein was measured using a fluorescence assay based on bovine serum albumin labeled with fluorescein. Acrylamide, 2-hydroxyethyl methacrylate, vinyl pyrrolidinone, and poly(ethylene glycol) methacrylate (PEGMA) monomers were grafted and evaluated for their ability to prevent protein adsorption. Photografted layers of PEGMA reduced protein adsorption to less than 2% relative to unmodified surfaces. The sequential two-step photografting process consisted in (i) the formation of covalently bound surface photoinitiator sites followed by (ii) surface-localized graft polymerization. Monomer concentration and irradiation time during photografting were found to be the most important parameters for optimization of the two-step process. For simultaneous single- step photografting, the solvent and the presence of photoinitiator were the key variables. Initiator-free single- step photografting was less efficient than the two-step technique, yet resulted in similar prevention of protein adsorption after grafting for an extended period of time. The utility of photografting as a simple, patternable modification technique was demonstrated by first creating a hydrophilic surface within the monolith that was subsequently patterned with a layer of reactive 2-vinyl-4,4-dimethylazlactone polymer chains followed by the immobilization of green fluorescent protein.