Interaction of the Carlavirus Cysteine-Rich Protein with the Plant Defense System
Interaction of the Carlavirus Cysteine-Rich Protein with the Plant Defense System
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DOI：
10.1007/s11008-005-0094-9
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发表时间：
2005-09
影响因子：
1.2
通讯作者：
N. Lukhovitskaya;A. Solovyev;T. E. Koshkina;S. Zavriev;S. Morozov
中科院分区：
文献类型：
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作者：
N. Lukhovitskaya;A. Solovyev;T. E. Koshkina;S. Zavriev;S. Morozov
卡拉病毒属病毒的基因组编码一种功能未知的小的富含半胱氨酸的蛋白质（CRP）。为了研究C反应蛋白在菊花病毒B（CVB）中的作用，构建了含有CVB C反应蛋白基因的重组马铃薯X病毒（PVX）基因组。CVB-CRP在PVX遗传环境中的表达显著改变了PVX在本氏烟草上引起的症状特征，重组病毒在本氏烟草上引起的症状是接种叶片的局部坏死性病变和顶端叶片坏死，而不是PVX在本氏烟草上引起的无症状感染和轻度花叶。客栈在烟草中，感染模式取决于植物宿主基因型：重组PVX仅在携带N基因的植物中系统传播。Agroinfiltration介导的瞬时表达分析表明，CRP既不作为一个无毒因子在N。本他明抑制转录后基因沉默。CVB CRP被鉴定为病毒致病性决定因子，其以依赖于植物防御的方式控制病毒与宿主植物的相互作用，所述植物防御由抗性基因如N基因介导。
The genome of viruses of the genusCarlaviruscodes for a small cysteine-rich protein (CRP) with unknown functions. To study the role of CRP of the chrysanthemum virus B (CVB), a recombinant potato virus X (PVX) genome containing the CVB CRP gene was constructed. Expression of CVB CRP in the PVX genetic surrounding drastically changed the character of symptoms produced by PVX inNicotiana benthamiana.The recombinant virus caused local necrotic lesions of inoculated leaves and necrosis of apical leaves rather than asymptomatic infection and mild mosaic, which are caused by PVX in this host plant. InN. tabacum, the infection pattern depended on the plant host genotype: recombinant PVX spread systemically only in plants carrying theNgene. Agroinfiltration-mediated transient expression assays showed that CRP neither acts as an avirulence factor inN. benthamiananor suppresses posttranscriptional gene silencing. CVB CRP was identified as a virus pathogenicity determinant that controls the interaction of the virus with the host plant in a way that depends on plant defense, which is mediated by resistance genes such as theNgene.