MAHRP2, an exported protein of Plasmodium falciparum, is an essential component of Maurer's cleft tethers
MAHRP2, an exported protein of Plasmodium falciparum, is an essential component of Maurer's cleft tethers
复制标题
MAHRP2 是恶性疟原虫的一种输出蛋白，是 Maurer 裂系绳的重要组成部分
作者：
Esther Pachlatko;Sebastian Rusch;A. Müller;A. Hemphill;L. Tilley;E. Hanssen;H. Beck
在侵入红细胞后，恶性疟原虫必须破坏宿主细胞。本研究的目的是阐明MAHRP 2在这些过程中的位置和功能。使用免疫荧光和免疫电子显微镜，我们发现膜相关的富组氨酸蛋白2（MAHRP 2）在此过程中被输出到红细胞胞质中的新圆柱形结构。我们假设这些结构将称为毛雷尔裂隙的细胞器拴在红细胞骨架上。表达MAHRP 2-GFP的寄生虫转染子的活细胞成像显示了系链样结构的移动的和固定的群体。差速离心允许这些新结构的富集。MAHRP 2既不具有信号肽也不具有PEXEL基序，并且使用表达截短的MAHRP 2片段的转染子来确定输出所需的序列。前15个氨基酸和富含组氨酸的N-末端区域对于MAHRP 2的正确运输以及预测的疏水区域是必需的。溶解研究表明MAHRP 2是膜结合的，但不是跨膜的。几次试图删除mahrp 2基因的尝试都失败了，这表明这种蛋白质对寄生虫的生存至关重要。
Upon invasion into erythrocytes, the malaria parasite Plasmodium falciparum must refurbish the host cell. The objective of this study was to elucidate the location and function of MAHRP2 in these processes. Using immunofluorescence and immunoelectron microscopy we showed that the membrane‐associated histidine‐rich protein‐2 (MAHRP2) is exported during this process to novel cylindrical structures in the erythrocyte cytoplasm. We hypothesize that these structures tether organelles known as Maurer's clefts to the erythrocyte skeleton. Live cell imaging of parasite transfectants expressing MAHRP2–GFP revealed both mobile and fixed populations of the tether‐like structures. Differential centrifugation allowed the enrichment of these novel structures. MAHRP2 possesses neither a signal peptide nor a PEXEL motif, and sequences required for export were determined using transfectants expressing truncated MAHRP2 fragments. The first 15 amino acids and the histidine‐rich N‐terminal region are necessary for correct trafficking of MAHRP2 together with a predicted hydrophobic region. Solubilization studies showed that MAHRP2 is membrane associated but not membrane spanning. Several attempts to delete the mahrp2 gene failed, indicating that the protein is essential for parasite survival.
编码新型高分子量宿主膜相关蛋白 PfEMP3 的恶性疟原虫基因的克隆和表征。
影响因子：
1.5
作者：
Pasloske,BL;Baruch,DI;vanSchravendijk,MR;Handunnetti,SM;Aikawa,M;Fujioka,H;Taraschi,TF;Gormley,JA;Howard,RJ
通讯作者：
Howard,RJ
影响因子：
3.6
作者：
Haase, Silvia;Herrmann, Susann;Spielmann, Tobias
通讯作者：
Spielmann, Tobias
恶性疟原虫感染的红细胞中红细胞细胞骨架和整合膜蛋白的超微结构定位。
影响因子：
6.6
作者：
Atkinson,CT;Aikawa,M;Perry,G;Fujino,T;Bennett,V;Davidson,EA;Howard,RJ
通讯作者：
Howard,RJ