Biocompatible surfactants for water-in-fluorocarbon emulsions
Biocompatible surfactants for water-in-fluorocarbon emulsions
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DOI：
10.1039/b806706f
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发表时间：
2008-10-01
期刊：
影响因子：
6.1
通讯作者：
Weitz, D. A.
中科院分区：
文献类型：
--
作者：
Holtze, C.;Rowat, A. C.;Weitz, D. A.
氟碳化合物包水乳液的液滴在体外和体内生物系统的区室化方面具有很大的潜力;然而，用于稳定这种乳液的表面活性剂是稀缺的。在这里，我们提出了一类新的含氟表面活性剂，我们合成的低聚全氟聚醚（PFPE）与聚乙二醇（PEG）的耦合。我们证明，这些嵌段共聚物表面活性剂稳定的水氟碳油乳液在所有必要的步骤的滴基实验，包括液滴形成，孵育，并重新注入到第二个微流体装置。此外，我们表明，与这些表面活性剂稳定的水滴可用于体外翻译（IVT），以及封装和孵育的单细胞。该乳液系统与生物系统和聚二甲基硅氧烷（PDMS）微流体装置的相容性使这些表面活性剂成为广泛的高通量液滴应用的理想选择。
Drops of water-in-fluorocarbon emulsions have great potential for compartmentalizing both in vitro and in vivo biological systems; however, surfactants to stabilize such emulsions are scarce. Here we present a novel class of fluorosurfactants that we synthesize by coupling oligomeric perfluorinated polyethers (PFPE) with polyethyleneglycol (PEG). We demonstrate that these block copolymer surfactants stabilize water-in-fluorocarbon oil emulsions during all necessary steps of a drop-based experiment including drop formation, incubation, and reinjection into a second microfluidic device. Furthermore, we show that aqueous drops stabilized with these surfactants can be used for in vitro translation (IVT), as well as encapsulation and incubation of single cells. The compatability of this emulsion system with both biological systems and polydimethylsiloxane (PDMS) microfluidic devices makes these surfactants ideal for a broad range of high-throughput, drop-based applications.