Anti-Müllerian hormone (AMH), inhibin-α, growth differentiation factor 9 (GDF9), and bone morphogenic protein-15 (BMP15) mRNA and protein are influenced by photoperiod-induced ovarian regression and recrudescence in Siberian hamster ovaries.
Anti-Müllerian hormone (AMH), inhibin-α, growth differentiation factor 9 (GDF9), and bone morphogenic protein-15 (BMP15) mRNA and protein are influenced by photoperiod-induced ovarian regression and recrudescence in Siberian hamster ovaries.
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西伯利亚仓鼠卵巢中抗苗勒氏管激素 (AMH)、抑制素-α、生长分化因子 9 (GDF9) 和骨形态发生蛋白 15 (BMP15) mRNA 和蛋白质受光周期诱导的卵巢退化和复发的影响。
DOI：
10.1002/mrd.22215
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发表时间：
2013
影响因子：
2.5
通讯作者：
Young,KellyA
中科院分区：
文献类型：
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作者：
Shahed,Asha;Young,KellyA
西伯利亚仓鼠暴露于短光周期（SD）会抑制卵巢功能，包括卵泡发生，而当它们转移到长光周期（LD）时，功能会恢复。为了研究光刺激复发的机制，我们评估了整个动情周期以及光衰退和复发卵巢中的关键卵泡生成因子——抗苗勒氏管激素（AMH）、抑制素-α、生长分化因子-9（GDF9）和骨形态发生蛋白-15（BMP15）。成年仓鼠暴露于 LD 或 SD 14 周，分别代表功能性卵巢和退化卵巢。选择回归的仓鼠被转移回 LD 2（转移后第 2 周；PTw2）或 8 周（PTw8）。收集卵巢并福尔马林固定进行免疫组化或液氮冷冻进行实时PCR。发情周期各阶段均检测AMH、抑制素α、GDF9和BMP15 mRNA和蛋白。与 LD 相比，14 周的 SD 暴露增加了 (P<0.05) 卵巢 AMH、GDF9 和 BMP15，但抑制素-α mRNA 水平没有增加。将退化仓鼠转移到刺激性长光周期 8 周，使 AMH 和 GDF9 mRNA 水平恢复到 LD 处理的水平，并进一步增加抑制素-α 和 BMP15 的 mRNA 水平。 AMH、抑制素-α、GDF9 和 BMP15 蛋白的免疫染色在窦前/窦卵泡和卵母细胞中最为强烈。 AMH 和抑制素-α 的整体免疫染色程度通常反映了 mRNA 数据，但 GDF9 或 BMP15 免疫染色没有观察到变化。不同光周期条件下 mRNA 和蛋白质水平的变化表明，这些卵泡生成因子通过对卵泡募集、保存和发育的潜在调节，在光刺激复发中可能发挥综合作用。重现。开发。 80: 895–907, 2013。© 2013 Wiley periodicals, Inc.
Exposure of Siberian hamsters to short photoperiod (SD) inhibits ovarian function, including folliculogenesis, whereas function is restored with their transfer to long photoperiods (LD). To investigate the mechanism of photo‐stimulated recrudescence, we assessed key folliculogenic factors–anti‐Müllerian hormone (AMH), inhibin‐α, growth differentiation factor‐9 (GDF9), and bone morphogenic protein‐15 (BMP15)–across the estrus cycle and in photo‐regressed and recrudescing ovaries. Adult hamsters were exposed to either LD or SD for 14 weeks, which respectively represent functional and regressed ovaries. Select regressed hamsters were transferred back to LD for 2 (post‐transfer week 2; PTw2) or 8 weeks (PTw8). Ovaries were collected and fixed in formalin for immunohistochemistry or frozen in liquid nitrogen for real‐time PCR.AMH, inhibin‐α,GDF9, andBMP15mRNA and protein were detected in all stages of the estrus cycle. Fourteen weeks of SD exposure increased (P< 0.05) ovarianAMH,GDF9, andBMP15, but not inhibin‐α mRNA levels as compared to LD. Transfer of regressed hamsters to stimulatory long photoperiod for 8 weeks returnedAMHandGDF9mRNA levels to LD‐treated levels, and further increased mRNA levels for inhibin‐α andBMP15. Immunostaining for AMH, inhibin‐α, GDF9, and BMP15 proteins was most intense in preantral/antral follicles and oocytes. The overall immunostaining extent for AMH and inhibin‐α generally mirrored the mRNA data, though no changes were observed for GDF9 or BMP15 immunostaining. Shifts in mRNA and protein levels across photoperiod conditions suggest possible syncretic roles for these folliculogenic factors in photo‐stimulated recrudescence via potential regulation of follicle recruitment, preservation, and development.Mol. Reprod. Dev. 80: 895–907, 2013. © 2013 Wiley Periodicals, Inc.
DOI：
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发表时间：
1987
期刊：
影响因子：
--
作者：
W. Reznikoff;C. Gross;R. Burgess;Record Mt;J. Dahlberg;M. Wickens
通讯作者：
M. Wickens