Rab13 Traffics on Vesicles Independent of Prenylation
Rab13 Traffics on Vesicles Independent of Prenylation
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DOI：
10.1074/jbc.m116.722298
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发表时间：
2016-05-13
影响因子：
4.8
通讯作者：
McPherson, Peter S.
中科院分区：
文献类型：
--
作者：
Ioannou, Maria S.;Girard, Martine;McPherson, Peter S.
Rab GTP酶是膜运输的关键调节剂。典型的观点是Rab在其非活性GDP结合形式中是可溶的，并且仅在活化和转化为其GTP结合状态时，它们通过C-末端异戊二烯基的膜插入锚定到膜上。在这里，我们表明，C-末端异戊烯化是不需要Rab 13与囊泡和交通。相反，无活性的Rab 13似乎通过蛋白质-蛋白质相互作用与囊泡结合。只有在激活后Rab 13才与质膜结合，推测是将C-末端异戊二烯基插入膜中。
Rab GTPases are critical regulators of membrane trafficking. The canonical view is that Rabs are soluble in their inactive GDP-bound form, and only upon activation and conversion to their GTP-bound state are they anchored to membranes through membrane insertion of a C-terminal prenyl group. Here we demonstrate that C-terminal prenylation is not required for Rab13 to associate with and traffic on vesicles. Instead, inactive Rab13 appears to associate with vesicles via protein-protein interactions. Only following activation does Rab13 associate with the plasma membrane, presumably with insertion of the C-terminal prenyl group into the membrane.