MMP-3 activation is a hallmark indicating an early change in TMJ disorders, and is related to nitration
MMP-3 activation is a hallmark indicating an early change in TMJ disorders, and is related to nitration
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DOI：
10.1016/j.ijom.2008.11.003
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发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Yoshimura, Y.
中科院分区：
文献类型：
--
作者：
Fujita, H.;Morisugi, T.;Yoshimura, Y.
近年来对颞下颌关节紊乱病的研究表明，基质金属蛋白酶（MMPs）与颞下颌关节内紊乱（ID）的病理生理过程密切相关。本研究的目的是探讨基质金属蛋白酶（MMP）在不同阶段的ID的滑液（SF）。为了研究MMP的激活和ID的进展之间的关系，54例来自ID患者的SIF样本的分类的基础上，Wilkes的标准和MMP活性测定。结果发现，MMP-3活性在中期短暂升高。MMP-3活性形式的这种增加也通过蛋白质印迹法证实。当54个样本被分为两组的基础上存在或不存在炎症的发现，MMP-3活性的强度与炎症症状。这些结果表明，MMP-3的激活是早期退行性变的ID的一个标志。泰乐菌素硝化过氧亚硝酸盐可以调节酶的活性。为了阐明MMPs在体内的活化途径，通过免疫沉淀分析SF中的硝化蛋白。SIF中的一些硝化蛋白被鉴定为MMP-2和MMP-3，并且MMP-3的硝化使它们在体外具有活性。
Recent studies oil temporomandibular joint (TMJ) disorders have suggested that matrix metalloproteinases (MMPs) are closely involved in the pathophysiological progression of the internal derangement (ID) of TMJ. The aim of this sutyd was to investigate MMPs in synovial fluid(SF) at different stages of ID. To examine the relationship between MMP activation and ID progression, 54 SIF samples from ID patients were classified based oil the criteria of Wilkes and were assayed for MMP activity. It was found that MMP-3 activity was transiently increased in the intermediate stage. This increase in the active form of MMP-3 was also confirmed by Western blotting. When the 54 samples were classified into two groups based on the presence or absence of inflammatory findings, the intensity of MMP-3 activity correlated with the inflammatory symptoms. These findings suggest that MMP-3 activation is a hallmark of early degenerative changes in ID. The tylosin nitration by the peroxynitrite can regulate the enzyme activity. To elucidate the activating pathway of MMPs in vivo, nitrated proteins in SF were analysed by immunoprecipitation. Some nitrated proteins in SIF were identified as MMP-2 and -3, and the nitration of MMP-3 rendered them active in vitro.