Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes.
Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes.
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DOI：
10.1167/iovs.06-1034
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发表时间：
2007-04
影响因子：
4.4
通讯作者：
Hangxiu Xu;D. D. Sta Iglesia-D.;Jennifer L. Kielczewski;Danielle F Valenta;M. Pease;D. Zack;H. Quigley
中科院分区：
文献类型：
--
作者：
Hangxiu Xu;D. D. Sta Iglesia-D.;Jennifer L. Kielczewski;Danielle F Valenta;M. Pease;D. Zack;H. Quigley
目的从成年哺乳动物睫状体中分离和鉴定睫状体祖细胞。方法：作者从成年小鼠、大鼠和人尸体眼的睫状体中分离祖细胞，并测定称为神经球（NS）细胞的祖细胞群的定量生长特征，包括小鼠、大鼠和人眼中单个细胞直径、NS直径、NS形成细胞的百分比和细胞数量。用共聚焦显微镜和透射电镜观察NS细胞的免疫标记和超微结构。结果培养1周后，小鼠、大鼠和人的单个细胞和神经球的平均直径相似（细胞直径：22 +/- 1.1，21 +/- 0.3，25 +/- 0.4 μ m; NS直径：139 +/- 22，137 +/- 9，141 +/- 11 μ m）。每个NS的平均细胞数估计为：小鼠1183个，大鼠5360个，人685个。在NS细胞中通过免疫标记鉴定的分子包括巢蛋白、Chx-10、波形蛋白、GFAP和Pax-6。部分NS细胞表达Thy-1。电镜下NS细胞粗面内质网丰富，突起较多，但无成熟神经元或胶质细胞的特征。结论：成年哺乳动物睫状体的祖细胞具有显著但有限的增殖潜力，并表达其他祖细胞的特征性标志物，并在视网膜发育早期观察到。睫状体可以作为实验性啮齿动物眼移植和人眼自体移植的细胞来源。
PURPOSE To isolate and characterize progenitor cells derived from adult mammalian ciliary body. METHODS The authors isolated progenitor cells from the ciliary body of adult mice, rats, and human cadaver eyes and determined quantitative growth characteristics of groups of progenitor cells called neurosphere (NS) cells, including individual cell diameter, NS diameter, percentage of NS-forming cells, and cell number per eye in mouse, rat, and human eyes. The immunolabeling and ultrastructure of NS cells were investigated by confocal and transmission electron microscopy. RESULTS Average diameters of individual cells and neurospheres after 1 week in culture were similar in mice, rats, and humans (cell diameters: 22 +/- 1.1, 21 +/- 0.3, 25 +/- 0.4 mum; NS diameters: 139 +/- 22, 137 +/- 9, 141 +/- 11 mum, respectively). Mean numbers of cells per NS were estimated to be 1183 in mice, 5360 in rats, and 685 in humans. Molecules that were identified by immunolabeling in NS cells included nestin, Chx-10, vimentin, GFAP, and Pax-6. Thy-1 was expressed in some NS cells. Ultrastructurally, NS cells displayed abundant rough endoplasmic reticulum and many cellular processes but no characteristics of mature retinal neurons or glia. CONCLUSIONS Progenitor cells from adult mammalian ciliary body have significant, but limited, proliferation potential and express markers characteristic of other progenitor cells and seen during early retinal development. The ciliary body could be a source of cells for transplantation in experimental rodent eyes and for autotransplantation in human eyes.