A genotypic and phenotypic comparison of type III secretion profiles of Pseudomonas aeruginosa cystic fibrosis and bacteremia isolates
A genotypic and phenotypic comparison of type III secretion profiles of Pseudomonas aeruginosa cystic fibrosis and bacteremia isolates
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DOI：
10.1016/j.ijmm.2007.02.004
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发表时间：
2007-07-01
影响因子：
4.1
通讯作者：
Curtis, Michael A.
中科院分区：
文献类型：
--
作者：
Wareham, David W.;Curtis, Michael A.
铜绿假单胞菌的 III 型分泌系统 (TTSS) 能够输送许多参与破坏真核上皮表面的毒素。虽然分泌 ExoS 的能力促进侵袭和内化，但 ExoU 的分泌介导急性细胞毒性。为了确定分泌这些毒素的能力与人类感染的性质和严重程度之间的任何关联，通过多重 PCR 和蛋白质印迹测定了 163 个临床分离株的 TTSS 基因型和表型。 exoS(+)/exoU(-) 基因型与囊性纤维化患者的慢性感染相关，而 exoS(-)/exoU(+) 基因型与从血液中分离的菌株相关。 ExoU 蛋白的分泌在从血液中获得的分离物中更常见，表明这种能力可能在急性侵袭性感染的发展中很重要。检测临床材料中的 TTSS 毒素可能有助于靶向抗菌治疗或识别感染侵袭性铜绿假单胞菌菌株的个体。 (C) 2007 爱思唯尔有限公司。版权所有。
The type III secretion system (TTSS) of Pseudomonas aeruginosa enables delivery of a number of toxins involved in the disruption of eukaryotic epithelial surfaces. Whilst the ability to secrete ExoS facilitates invasion and internalization, the secretion of ExoU mediates acute cytotoxicity. In order to determine any association with the ability to secrete these toxins with the nature and severity of human infection, the TTSS genotypes and phenotypes of 163 clinical isolates were determined by multiplex PCR and Western blotting. An exoS(+)/exoU(-) genotype was associated with chronic infection in patients with cystic fibrosis whilst an exoS(-)/exoU(+) genotype was associated with strains isolated from blood. Secretion of the ExoU protein was more commonly seen in isolates obtained from blood, suggesting this ability may be important in the development of acute invasive infection. Detection of TTSS toxins in clinical material may be useful in targeting antimicrobial therapy or identifying individuals infected with aggressive strains of P. aeruginosa. (C) 2007 Elsevier GmbH. All rights reserved.