Degenerating Processes Identified by Electron Microscopic lmmunocytochemical Methods
Degenerating Processes Identified by Electron Microscopic lmmunocytochemical Methods
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DOI：
10.1007/978-1-61779-328-8_3
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Pierce, Joseph P.
中科院分区：
文献类型：
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作者：
Milner, Teresa A.;Waters, Elizabeth M.;Pierce, Joseph P.
电子显微镜免疫标记技术在神经组织退化过程的识别和分析中的应用极大地增强了研究人员检查细胞凋亡和其他退行性疾病机制的能力。对于此类机制的早期阶段尤其如此。传统上，只有在发生显着的细胞萎缩后，当亚细胞细节变得模糊并且突触关系发生改变时，才能在超微结构水平上识别退化过程。使用免疫细胞化学标记程序，首先通过使用针对各种退行性标志物的抗体来识别退行性神经和 OM 过程，例如促凋亡效应物（即细胞质细胞色素 c）、病理成分（即 β 淀粉样蛋白沉积物）或炎症因子（即 Ibal）。然后可以仔细表征过程内标记的亚细胞分布以及标记过程与周围元素的关系。获得的信息可以通过使用双重免疫标记进一步细化，这可以提供关于退化过程的表型和过程输入的额外数据。
The application of electron microscopic immunolabeling techniques to the identification and analysis of degenerating processes in neural tissue has greatly enhanced the ability of researchers to examine apoptosis and other degenerative disease mechanisms. This is particularly true for the early stages of such mechanisms. Traditionally, degenerating processes could only be identified at the ultrastructural level after significant cellular atrophy had occurred, when subcellular detail was obscured and synaptic relationships altered. Using immunocytochemical labeling procedures, degenerating neural and OM processes arc first identified through the use of antibodies directed against a variety of degenerative markers, such as proapoptotic effectors (i.e., cytoplasmic cytochrome c), pathological components (i.e., beta amyloid deposits), or inflammatory agents (i.e., Ibal). Both the subcellular distribution of the marker within the process and the relationship of the labeled process to surrounding elements can then be carefully characterized. The information obtained can be further refined through the use of dual immunolabeling, which can provide additional data on the phenotype of the degenerating process and inputs to the process.