Proteomic Analysis of Rice Seedlings Under Cold Stress
Proteomic Analysis of Rice Seedlings Under Cold Stress
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DOI：
10.1007/s10930-017-9721-2
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发表时间：
2017-08-01
期刊：
影响因子：
3
通讯作者：
Qiu, Yongfu
中科院分区：
文献类型：
--
作者：
Ji, Li;Zhou, Ping;Qiu, Yongfu
低温对水稻的生长发育有很大的制约作用。水稻幼苗在低温条件下生长迟缓，叶片缠绕，部分叶片甚至死亡。为了更好地了解水稻对低温胁迫的反应，采用双向电泳技术和质谱生物信息学分析技术，对低温胁迫下水稻冷敏品系9311和抗寒品种藤坂5号的耐冷性进行了初步研究。建立了低温处理下9311和藤坂5号的双向电泳图谱。通过生物信息学分析，发现这些蛋白质参与水稻发育的许多方面。最大的一类蛋白质是在新陈代谢中起作用的。通过比较两个品种的蛋白质，发现9311的大部分蛋白质表达下调，而藤坂5号的大部分蛋白质表达上调。结果表明，低温胁迫下，水稻细胞膜组成和结构受到破坏，代谢发生显著变化，防御系统被激活。本研究共鉴定了59个与植物抗冷胁迫相关的蛋白质，并对其生物学功能进行了分类。本研究的意义在于为水稻抗冷性的研究提供一定的指导，为水稻品种的选育提供辅助技术支持，拓宽植物耐冷性的研究领域。
Low temperature can greatly restrict the growth and development of rice. The rice seedlings show growth retardation, lamina wrap, and part of blade even died under the condition of low temperature. In order to get more information about cold stress responses in rice, two dimensional electrophoresis and bioinformatics analysis of mass spectrometry were used to preliminary survey the cold tolerance of cold sensitive line 9311 and cold resistance variety Fujisaka 5 under cold stress. Two dimensional electrophoresis maps of 9311 and Fujisaka 5 were established under cold treatment. With analysis of bioinformation, the proteins were found involve in many aspects of rice development. The largest category of proteins is functioning on metabolism. By comparing the proteins from the two varieties, it can be found that most proteins from 9311 were down-regulated and were up-regulated in Fujisaka 5. The results showed that the membrane composition and structure were damaged, metabolism changed dramatically and rice defense system was activated under the cold stimulation. Fifty-nine proteins related to the resistance of cold stress were identified in our study, and we have investigated and classified all of their biological functions. The importance of our study are providing some conduct for the research of rice resistant to cold stress, supporting auxiliary technique for rice varieties and widening the search field of cold tolerance in plants.