Innate immune activation and antitumor effects of Lactobacillus-fermented Sparassis crispa extract in mice
Innate immune activation and antitumor effects of Lactobacillus-fermented Sparassis crispa extract in mice
复制标题
DOI：
10.1016/j.jff.2020.104215
复制
发表时间：
2020-12
影响因子：
5.6
通讯作者：
K. Hiramoto;J. Nishioka;Koji Suzuki
中科院分区：
文献类型：
--
作者：
K. Hiramoto;J. Nishioka;Koji Suzuki
松萝（SC）是一种富含β（1-3）-葡聚糖的蘑菇，具有激活天然免疫的作用。乳酸菌也被称为激活免疫力。本文研究了乳酸杆菌发酵SC（Lactobacillus-fermented SC，LFSC）和SC对小鼠天然免疫和肿瘤生长的影响。LFSC处理比SC处理促进更高的先天免疫细胞的积累。然而，LFSC的抗肿瘤活性低于氟尿嘧啶（5-FU）。LFSC还能显著降低小鼠血清中IL-6的水平，提高小鼠肿瘤组织的凋亡率和活性氧的产生。在5-FU处理的小鼠中未观察到这些作用。LFSC还显著增强人单核细胞中血栓/炎症因子的吞噬活性和mRNA表达。这些结果表明，LFSC显着增强天然免疫，并通过不同于5-FU的机制表现出抗肿瘤作用。
Sparassis crispa(SC), a mushroom abundantly containing β(1–3)-glucan, is known to activate the innate immunity. Lactic acid bacteria are also known to activate the immunity. Here, we investigated the effects of SC andLactobacillus-fermented SC (LFSC) on the innate immunity and tumor growth in mice. LFSC treatment promoted the higher accumulation of innate immune cells than the SC treatment. However, the antitumor activity of the LFSC was lower than that of the fluorouracil (5-FU). Furthermore, LFSC significantly reduced the serum levels of interleukin-6 in mice and enhanced the apoptosis rate and the production of reactive oxygen species in mouse tumor tissues. These effects were not observed in 5-FU-treated mice. LFSC also significantly enhanced the phagocytic activity of and the mRNA expression of thrombotic/inflammatory factors in human monocytes. These findings suggest that LFSC significantly enhances the innate immunity and exhibits antitumor effects by a mechanism different from that of 5-FU.