Interactions between isolated nucleosome core particles: A tail-bridging effect?
Interactions between isolated nucleosome core particles: A tail-bridging effect?
复制标题
分离的核小体核心颗粒之间的相互作用：尾桥效应？
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
F. Livolant
中科院分区：
文献类型：
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作者：
S. Mangenot;E. Raspaud;C. Tribet;L. Belloni;F. Livolant
翻译后摘要：孤立的核小体核心颗粒之间的相互作用进行了研究，作为一个功能的一价盐浓度的渗透压和电泳迁移率测量。将数据与对无蛋白质DNA片段进行的测量进行比较，并使用常规理论方法进行分析。在低盐，静电屏蔽效应占第二维里系数的变化，而简单的硬核贡献成为主要在高盐。在中间范围内，吸引力发生。根据先前的结果（Mangenot等人，Biophys. J. 82，345（2002）），我们证明了柔性的基本蛋白质尾部负责这种吸引。讨论了尾桥效应。
Abstract:Interactions between isolated nucleosome core particles are studied as a function of the monovalent salt concentration by osmometry and by electrophoretic mobility measurements. The data are compared to the measurements performed on the protein-free DNA fragments and also analysed using the conventional theoretical approach. At low salt, an electrostatic screening effect accounts for the variation of the second virial coefficient whereas the simple hard-core contribution becomes predominant at high salt. In the intermediate range, an attraction occurs. In the light of previous results (Mangenot et al. Biophys. J. 82, 345 (2002)), we show that the flexible basic proteic tails are responsible for this attraction. A tail-bridging effect is discussed.