Lifespan decrease in a Caenorhabditis elegans mutant lacking TRX-1, a thioredoxin expressed in ASJ sensory neurons
Lifespan decrease in a Caenorhabditis elegans mutant lacking TRX-1, a thioredoxin expressed in ASJ sensory neurons
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DOI：
10.1016/j.febslet.2005.12.046
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发表时间：
2006-01-23
期刊：
影响因子：
3.5
通讯作者：
Swoboda, P
中科院分区：
文献类型：
--
作者：
Miranda-Vizuete, A;González, JCF;Swoboda, P
硫氧还蛋白是一类在调节许多细胞氧化还原过程中起关键作用的小蛋白。我们在这里报告了后生动物中主要与神经元相关的硫氧还蛋白家族的第一个成员的特征。秀丽隐杆线虫基因B0228.5编码一种硫氧还蛋白（TRX-1），该蛋白在ASJ纤毛感觉神经元中表达，在一定程度上也在大多数后肠细胞中表达。TRX-1在异源硫氧还蛋白还原酶存在下具有还原蛋白二硫化物的活性。与野生型相比，携带trx-1基因空等位基因的突变型蠕虫在平均寿命和最长寿命方面均表现出可重复的减少。TRX-1的鉴定和表征为以秀丽隐杆线虫为体内模型研究硫氧还毒素在寿命和神经系统生理病理中的作用铺平了道路。(c) 2005年欧洲生化学会联合会。Elsevier B.V.版权所有。
Thioredoxins are a class of small proteins that play a key role in regulating many cellular redox processes. We report here the characterization of the first member of the thioredoxin family in metazoans that is mainly associated with neurons. The Caenorhabditis elegans gene B0228.5 encodes a thioredoxin (TRX-1) that is expressed in ASJ ciliated sensory neurons, and to some extent also in the posterior-most intestinal cells. TRX-1 is active at reducing protein disulfides in the presence of a heterologous thioredoxin reductase. A mutant worm strain carrying a null allele of the trx-1 gene displays a reproducible decrease in both mean and maximum lifespan when compared to wild-type. The identification and characterization of TRX-1 paves the way to use C elegans as an in vivo model to study the role of thioredoxins in lifespan and nervous system physiology and pathology. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.