Effect of Protein Binding Coverage, Location, and Distance on the Localized Surface Plasmon Resonance Response of Purified Au Nanoplates Grown Directly on Surfaces
Effect of Protein Binding Coverage, Location, and Distance on the Localized Surface Plasmon Resonance Response of Purified Au Nanoplates Grown Directly on Surfaces
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DOI：
10.1021/jp2010869
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发表时间：
2011-04-21
影响因子：
3.7
通讯作者：
Zamborini, Francis P.
中科院分区：
文献类型：
--
作者：
Beeram, Srinivas R.;Zamborini, Francis P.
本文报道了蛋白质结合覆盖位置和结合距离对直接生长在表面上的纯化金纳米板的局部表面等离子体共振（LSPR）响应的影响。纳米板表面对人抗igg结合的反应很大程度上取决于这些变量。抗igg附着法控制结合位置平台、边缘；（或顶点位点）和连接体覆盖和链长分别控制抗体覆盖和结合距离。例如，在超声纯化的纳米板样品上，中覆盖层、低覆盖层边缘位点和中覆盖层边缘位点结合的λ (max) （δ λ (max)）的平均值分别为21 +/- 5、44 +/- 2和53 +/- 4 nm。这种趋势与通过胶带净化的样品相似，除了由于这些样品的初始lambda（max）较小，移位都较小。原子力显微镜（AFM）图像显示了抗igg在纳米板上的覆盖范围和结合位置，并与LSPR光谱相关联，证实了边缘位点对蛋白质结合更敏感，δ λ (max)随抗igg覆盖范围的增加而增加。LSPR响应通常随着连接体链长度的减小而增加，并且在大多数情况下与理论预测非常吻合，尽管也有一些例外。具体来说，LSPR位移的增加顺序是巯基丙酸（MPA）、11-巯基癸酸（MUA）、16-巯基癸酸（MHDA）作为抗igg的连接体。例如，在超声纯化的Au纳米板上，抗igg附着在高覆盖边缘的平均δ λ (max)分别为61 +/- 5、53 +/- 4和42 +/- 3 nm。
Here we report the effect of protein binding coverage location, and binding distance on the localized surface Plasmon resonance (LSPR) response of purified Au nanoplates grown directly on surfaces. The response to human anti-IgG binding on the nanoplate surface depends strongly on these variables. The method of anti-IgG attachment Controlled the binding location terrace,, edge; or vertex sites) and the linker coverage and chain length controlled the antibody coverage and binding distance, respectively. The average, Change in lambda(max) (Delta lambda(max)) for binding to terraces at medium coverage, edge sites at low coverage and edge sites at medium Coverage was 21 +/- 5, 44 +/- 2, and 53 +/- 4 nm on nanoplate samples purified by sonication for example. The trend was similar for samples purified by taping, except that the shifts were all smaller due to a smaller initial lambda(max) for those samples. Atomic force microscopy (AFM) images reveal the coverage and binding location of anti-IgG on the nanoplates and, when correlated with the LSPR spectra, confirm that the edge sites are more sensitive to protein binding and Delta lambda(max) increases with increasing anti-IgG coverage. The LSPR response generally increases as the chain length of the linker decreases and matches quite well with that predicted by theory in most cases, although there are some exceptions. Specifically, the LSPR shift increases in the order of mercaptopropionic,acid (MPA) > 11-mercaptoundecanoic acid (MUA) > 16-mercaptohexadecanoic acid (MHDA) as linkers for anti-IgG. The average Delta lambda(max) for anti-IgG attached to the edge sites at high coverage on Au nanoplates purified by sonication Was 61 +/- 5, 53 +/- 4, and 42 +/- 3 nm, for the three linkers, respectively, for example.