Subgingival microbiota in healthy, well-maintained elder and periodontitis subjects
Subgingival microbiota in healthy, well-maintained elder and periodontitis subjects
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DOI：
10.1111/j.1600-051x.1998.tb02454.x
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发表时间：
1998-05-01
影响因子：
6.7
通讯作者：
Socransky, SS
中科院分区：
文献类型：
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作者：
Haffajee, AD;Cugini, MA;Socransky, SS
本研究比较了30名牙周健康（平均年龄36+/-9岁）、35名牙周组织维持良好的老年人（平均年龄77+/-5岁）和138名成人牙周炎患者（平均年龄46+/-11岁）的40种龈下菌种的部位患病率。203名受试者在基线时从每颗牙齿的内侧（多达28个样本）采集牙龈下菌斑样本。利用全基因组DNA探针和棋盘格DNA-DNA杂交技术测定了5003个牙菌斑样本中40个龈下分类群的存在和水平。临床评估包括牙龈红肿、探诊出血、菌斑积累和化脓的双重测量，以及每颗牙齿6个部位的袋深和附着水平的重复测量。计算每个受试者中每个物种定殖点的百分比（流行率）。使用Kruskal-Wallis测试寻找各组之间患病率和水平的差异。常见检出的放线菌2基因种、血链球菌和口腔链球菌在各被试组间差异不显著。经多重比较调整后，牙周炎组有4种菌种显著升高，且发病率更高。连翘拟杆菌定殖位点（+/-SEM）的平均%分别为10+/-3、12+/-2和40+/-2 (p
This investigation compared the site prevalence of 40 subgingival species in 30 periodontally healthy (mean age 36+/-9 years), 35 elders with a well-maintained periodontium (mean age 77+/-5) and 138 adult periodontitis subjects (mean age 46+/-11). Subgingival plaque samples were taken from the mesial aspect of each tooth (up to 28 samples) in the 203 subjects at baseline. The presence and levels of 40 subgingival taxa mere determined in 5003 plaque samples using whole genomic DNA probes and checkerboard DNA-DNA hybridization. Clinical assessments including dichotomous measures of gingival redness, bleeding on probing, plaque accumulation and suppuration, as well as duplicate measures of pocket depth and attachment level, were made at 6 sites per tooth. The % of sites colonized by each species (prevalence) was computed for each subject. Differences in prevalence and levels among groups were sought using the Kruskal-Wallis test. Commonly detected species, such as Actinomyces naeslundii genospecies 2, Streptococcus sanguis and Streptococcus oralis did not differ significantly among subject groups. After adjusting for multiple comparisons, 4 species were significantly elevated and at greater prevalence in the periodontitis group. Mean % of sites (+/-SEM) colonized by Bacteroides forsythus was 10+/-3, 12+/-2 and 40+/-2 (p