Differential Response and Priming Dose Effect on the Proteome of Human Fibroblast and Stem Cells Induced by Exposure to Low Doses of Ionizing Radiation
Differential Response and Priming Dose Effect on the Proteome of Human Fibroblast and Stem Cells Induced by Exposure to Low Doses of Ionizing Radiation
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DOI：
10.1667/rr14226.1
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发表时间：
2016-03-01
影响因子：
3.4
通讯作者：
Hornhardt, Sabine
中科院分区：
文献类型：
--
作者：
Hauptmann, Monika;Haghdoost, Siamak;Hornhardt, Sabine
有人提出，从机理上了解细胞对低剂量和剂量率的反应，可能有助于减少目前对癌症和非癌症相关辐射效应的风险估计所涉及的一些不确定性，这些不确定性是在线性无阈值假设中继承的。在这项研究中，低剂量辐射对人类成纤维细胞和干细胞蛋白质组的影响进行了研究。特别强调审查：1。在低剂量范围（40-140 mGy）的低线性能量转移（LET）辐射中蛋白质差异表达的剂量-反应关系;以及2.在激发剂量之前给予的引发剂量对蛋白质差异表达的影响（适应性反应效应）。这些研究是在培养的人成纤维细胞（VH 10）和人脂肪源性干细胞（ADSC）上进行的。VH 10细胞实验的结果表明，低剂量的低LET辐射诱导的差异表达的蛋白质的独特模式，为每个剂量的研究。此外，低引发辐射剂量显着改变蛋白质表达诱导随后的挑战曝光。在ADSC中，差异表达蛋白的数量明显少于VH 10细胞，表明ADSC对低剂量辐射的内在反应不同。蛋白质组学结果进一步讨论了低剂量辐射影响的可能途径。(C)2016辐射研究学会
It has been suggested that a mechanistic understanding of the cellular responses to low dose and dose rate may be valuable in reducing some of the uncertainties involved in current risk estimates for cancer- and non-cancer-related radiation effects that are inherited in the linear nothreshold hypothesis. In this study, the effects of low-dose radiation on the proteome in both human fibroblasts and stem cells were investigated. Particular emphasis was placed on examining: 1. the dose-response relationships for the differential expression of proteins in the low-dose range (40-140 mGy) of low-linear energy transfer (LET) radiation; and 2. the effect on differential expression of proteins of a priming dose given prior to a challenge dose (adaptive response effects). These studies were performed on cultured human fibroblasts (VH10) and human adipose-derived stem cells (ADSC). The results from the VH10 cell experiments demonstrated that low-doses of low-LET radiation induced unique patterns of differentially expressed proteins for each dose investigated. In addition, a low priming radiation dose significantly changed the protein expression induced by the subsequent challenge exposure. In the ADSC the number of differentially expressed proteins was markedly less compared to VH10 cells, indicating that ADSC differ in their intrinsic response to low doses of radiation. The proteomic results are further discussed in terms of possible pathways influenced by low-dose irradiation. (C) 2016 by Radiation Research Society