The effects of 8DSS peptide on remineralization in a rat model of enamel caries evaluated by two nondestructive techniques
The effects of 8DSS peptide on remineralization in a rat model of enamel caries evaluated by two nondestructive techniques
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通过两种无损技术评估 8DSS 肽对牙釉质龋齿大鼠模型再矿化的影响
DOI：
10.1177/2280800019827798
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发表时间：
2019-02-01
影响因子：
2.5
通讯作者：
Zhang, Linglin
中科院分区：
文献类型：
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作者：
Zheng, Wenyue;Ding, Longjiang;Zhang, Linglin
如今，龋齿是最常见的口腔健康问题之一，影响着大多数人。研究发现，在牙釉质龋形成的早期阶段，通过牙釉质再矿化，可以有效地保护牙齿免受龋齿的侵害。本研究将一种具有8个重复序列的天冬氨酸-丝氨酸-丝氨酸（8DSS）肽作为生物矿化剂应用于活体大鼠牙釉质龋模型。非破坏性定量光诱导荧光数字成像（QLF-D）和微型计算机断层扫描（micro-CT）用于评估牙釉质龋齿病变的再矿化，方法是测量使用QLF-D成像获得的臼齿区域的总荧光辐射损失（ΔQTotal），以及使用微型ct获得的矿物质密度和残留的臼齿牙釉质体积。ΔQTotal与矿物密度（强相关，r = 0.8000, p < 0.001）、ΔQTotal与残留磨牙釉质体积（中度相关，r = 0.6375, p < 0.001）之间存在相关性。我们的研究结果表明，8DSS是一种很有前景的体内再矿化剂，其效果与NaF相当（p < 0.05），这已通过经典的Keyes方法得到验证。此外，无损QLF-D和micro-CT方法可以在体内三维定量牙釉质龋齿病变的再矿化，在硬组织定量中具有广泛的应用价值。
Nowadays, dental caries is one of the most common oral health problems, affecting most individuals. It has been found that, by remineralizing enamel at an early stage in the formation of enamel caries, teeth can be effectively protected from dental caries. In this work, a peptide with eight repetitive sequences of aspartate-serine-serine (8DSS) is applied as the bio-mineralizer in an in-vivo rat enamel caries model. Nondestructive quantitative light-induced fluorescence-digital (QLF-D) imaging and micro-computed tomography (micro-CT) are used to evaluate the remineralization of enamel carious lesions by measuring the total fluorescence radiance loss of the molar area (ΔQTotal), acquired using QLF-D imaging, and the mineral density and residual molar enamel volume, acquired using micro-CT. Correlations are explored between ΔQTotal and mineral density (strong correlation, r = 0.8000, p < 0.001) and ΔQTotal and residual molar enamel volume (moderate correlation, r = 0.6375, p < 0.001). Our results demonstrate that 8DSS is a promising in-vivo remineralization agent that exhibits comparable effects to NaF (p < 0.05), which has been verified using the classical Keyes method. Moreover, the nondestructive QLF-D and micro-CT methods can be combined to quantify the remineralization of enamel carious lesions three-dimensionally in vivo, making them broadly applicable in quantifying hard tissues.