Upregulated circular RNA circ_0025033 promotes papillary thyroid cancer cell proliferation and invasion via sponging miR-1231 and miR-1304
Upregulated circular RNA circ_0025033 promotes papillary thyroid cancer cell proliferation and invasion via sponging miR-1231 and miR-1304
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DOI：
10.1016/j.bbrc.2019.01.108
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发表时间：
2019-03-05
影响因子：
3.1
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
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作者：
Pan, Yanhui;Xu, Tingting;Zhang, Wei
最近的证据表明，环状 RNA (circRNA) 是肿瘤发生的关键调节因子。然而，它们在甲状腺乳头状癌（PTC）中的作用尚不完全清楚。目前工作中，通过circRNA微阵列筛选PTC组织与癌旁非癌组织样本之间差异表达的circRNA。我们进一步选择肿瘤组织中表达最高的circRNA（circ_0025033）进行研究。使用 qRT-PCR 测量 PTC 组织样本和细胞系中的 circ_0025033 水平。进行功能获得/丧失测定以确定circ_0025033对细胞活力、克隆形成、细胞凋亡、迁移和侵袭的影响。进行双荧光素酶报告基因检测以研究 PTC 中 circ_0025033 的机制。数据显示，circ_0025033加剧细胞增殖、迁移和侵袭，抑制细胞凋亡。此外，circ_0025033可以直接在PTC细胞中吸收miR-1231和miR-1304。此外，circ_0025033 的致癌作用取决于其对 miR-1231 和 miR-1304 的抑制。总的来说，这项工作揭示了 circ_0025033/miR-1231/miR-1304 参与 PTC 启动和进展的新信号。 (C) 2019 Elsevier Inc. 保留所有权利。
Recent evidence revealed that circular RNAs (circRNAs) are key regulators for tumorigenesis. However, their roles in papillary thyroid cancer (PTC) is not fully understood. In the current work, the differentially expressed circRNAs between PTC tissues and adjacent noncancerous tissue samples were screened by circRNA microarray. We further selected the highest expressed circRNA (circ_0025033) in tumorous tissues for the study. qRT-PCR was used to measure the level of circ_0025033 in PTC tissue samples and cell lines. Gain/loss of function assays were carried out to determine the effect of circ_0025033 on cell viability, clone-forming, apoptosis, migration and invasion. Dual-luciferase reporter assays were conducted to investigate the mechanisms of circ_0025033 in PTC. The data showed that circ_0025033 aggravated cell proliferation, migration and invasion and inhibited cell apoptosis. Additionally, circ_0025033 could directly sponge miR-1231 and miR-1304 in PTC cells. Furthermore, the oncogenic role of circ_0025033 is dependent on its suppression of miR-1231 and miR-1304. Collectively, this work uncovers a novel signal of circ_0025033/miR-1231/miR-1304 involved in PTC initiation and progression. (C) 2019 Elsevier Inc. All rights reserved.