Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1

Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1
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使用 Acidovorax sp BoFeN1 模型系统进行 SEM 成像和分析的细菌细胞-矿物质聚集体的制备方法比较

DOI:
10.1080/01490451.2016.1189467
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发表时间:
2017
影响因子:
2.3
通讯作者:
Obst M.
Obst M.
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Zeitvogel F;Burkhardt CJ;Schroeppel B;Schmid G;Ingino P;Obst M.

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创新的微量分析方法是地球微生物学中有价值的工具。它们通常需要使用干燥的样品,这对样品制备提出了挑战。由于地质微生物样品通常具有强烈的异质性组成,因此选择制备方法并不简单。因此,我们比较了不同的干燥方法(临界点干燥,六甲基二硅氮烷干燥,空气干燥和冷冻干燥)如何影响细菌细胞矿物聚集体的结构。每种方法都被证明适用于特定目的,但没有一种方法能够完全保留样品结构。通过溅射涂层和树脂包埋过程中细胞外聚合物的保存获得了关于表面改变的更多信息。
Innovative microanalytical methods are valuable tools in geomicrobiology. They often require the use of dried samples, demanding a challenging sample preparation. Since geomicrobiological samples typically have a strongly heterogeneous composition, choosing a preparation method is not straightforward. We therefore compared how different drying methods (critical point drying, hexamethyldisilazane drying, air drying and freeze drying) influence the structure of bacterial cell-mineral aggregates. Each method proved suitable for a specific purpose, but none was able to completely preserve the sample structure. Additional information was obtained on surface alterations by sputter coating and on preservation of extracellular polymeric substances during resin embedding.
DOI: 10.1111/gbi.12019
发表时间: 2013-03-01
期刊: GEOBIOLOGY
影响因子: 3.7
作者:
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通讯作者: Kappler, A.
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Ludwig Reimer:Elektronenmikroskopische Untersuchungs‐ und Präparationsmethoden,erweiterte Auflage,柏林,海德堡,纽约 1967 年。598 Seiten,247 Abbildungen。
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