Myoblast differentiation on graphene oxide
Myoblast differentiation on graphene oxide
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DOI：
10.1016/j.biomaterials.2012.11.052
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发表时间：
2013-03-01
期刊：
影响因子：
14
通讯作者：
Park, Chan Beum
中科院分区：
文献类型：
--
作者：
Ku, Sook Hee;Park, Chan Beum
石墨烯基纳米材料在药物/基因传递、癌症治疗、成像和组织工程等生物医学领域的应用受到了广泛的关注。尽管二维碳材料是一种无毒的可植入平台，但它们对肌源性分化的影响却很少被研究。我们研究了石墨烯基纳米材料，特别是氧化石墨烯（GO）和还原氧化石墨烯（rGO）的肌管形成。将氧化石墨烯薄片固定在胺改性玻璃上制备氧化石墨烯改性玻璃，再经肼处理进一步还原制备氧化石墨烯改性底物。我们研究了小鼠成肌细胞C2C12在未修饰、氧化石墨烯修饰和反氧化石墨烯修饰的玻璃基质上的粘附、增殖和分化行为。根据我们对肌原性蛋白表达、多核肌管形成和分化特异性基因（MyoD、肌原素、肌钙蛋白T和MHC）表达的分析，氧化石墨烯显著增强了肌原性分化，这是由于血清蛋白吸附和纳米地形提示所致。我们的研究结果证明了氧化石墨烯刺激肌源性分化的能力，显示了骨组织工程应用的潜力。(C) 2012 Elsevier Ltd.版权所有。
Graphene-based nanomaterials have received much attention in biomedical applications for drug/gene delivery, cancer therapy, imaging, and tissue engineering. Despite the capacity of 2D carbon materials as a nontoxic and implantable platform, their effect on myogenic differentiation has been rarely studied. We investigated the myotube formation on graphene-based nanomaterials, particularly graphene oxide (GO) and reduced graphene oxide (rGO). GO sheets were immobilized on amine-modified glass to prepare GO-modified glass, which was further reduced by hydrazine treatment for the synthesis of rGO-modified substrate. We studied the behavior, including adhesion, proliferation, and differentiation, of mouse myoblast C2C12 on unmodified, GO-, and rGO-modified glass substrates. According to our analyses of myogenic protein expression, multinucleate myotube formation, and expression of differentiation-specific genes (MyoD, myogenin, Troponin T, and MHC), myogenic differentiation was remarkably enhanced on GO, which resulted from serum protein adsorption and nanotopographical cues. Our results demonstrate the ability of GO to stimulate myogenic differentiation, showing a potential for skeletal tissue engineering applications. (C) 2012 Elsevier Ltd. All rights reserved.