Localization of cholinergic differentiation factor/leukemia inhibitory factor mRNA in the rat brain and peripheral tissues.
Localization of cholinergic differentiation factor/leukemia inhibitory factor mRNA in the rat brain and peripheral tissues.
复制标题
胆碱能分化因子/白血病抑制因子 mRNA 在大鼠脑和外周组织中的定位。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
T. Yamamori
中科院分区：
文献类型：
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作者：
T. Yamamori
交感神经元在体外和体内表达的神经递质和神经肽表型表现出相当大的可塑性。胆碱能分化因子（CDF，也称为白血病抑制因子，LIF）诱导培养的大鼠交感神经元变得胆碱能，而不影响其生存或生长。为了了解该因子在正常发育中的作用，有必要确定它的原位产生位置。为了定位 CDF/LIF mRNA，采用半定量逆转录聚合酶链式反应方法。使用肌动蛋白和微管蛋白 mRNA 作为内部对照，比较两套不同的 CDF/LIF 引物。在出生后的大鼠外周组织中，CDF/LIF mRNA 选择性地定位于发育中的交感胆碱能神经元的目标区域；在交感去甲肾上腺素能神经元靶标中未检测到 mRNA。这一发现支持 CDF/LIF 是靶源性神经元分化因子的假设。在出生后的大鼠大脑中，CDF/LIF mRNA选择性地定位于视觉系统的两个部分：视觉皮层和上丘。因此，CDF/LIF 也可能在该系统中发挥作用。
Sympathetic neurons display considerable plasticity in the neurotransmitter and neuropeptide phenotypes they express in vitro and in vivo. The cholinergic differentiation factor (CDF, also known as leukemia inhibitory factor, LIF) induces cultured rat sympathetic neurons to become cholinergic, without affecting their survival or growth. To understand the role of this factor in normal development, it is essential to determine where it is produced in situ. To localize CDF/LIF mRNA, a semiquantitative, reverse transcription-polymerase chain reaction method was employed. Actin and tubulin mRNA were used as internal controls, and two different sets of CDF/LIF primers were compared. In postnatal rat peripheral tissues, CDF/LIF mRNA was selectively localized in the target area of developing, sympathetic cholinergic neurons; the mRNA was not detected in the targets of sympathetic noradrenergic neurons. This finding supports the hypothesis that CDF/LIF is a target-derived neuronal differentiation factor. In postnatal rat brain, CDF/LIF mRNA is localized selectively in two parts of the visual system, visual cortex and superior colliculus. Thus, CDF/LIF may play a role in this system as well.