Analysis of the affinity of influenza A virus protein epitopes for swine MHC I by a modified in vitro refolding method indicated cross-reactivity between swine and human MHC I specificities
Analysis of the affinity of influenza A virus protein epitopes for swine MHC I by a modified in vitro refolding method indicated cross-reactivity between swine and human MHC I specificities
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通过改良的体外重折叠方法分析甲型流感病毒蛋白表位对猪 MHC I 的亲和力，表明猪和人 MHC I 特异性之间存在交叉反应
DOI：
10.1007/s00251-018-1070-6
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发表时间：
2018-07
期刊：
影响因子：
3.2
通讯作者：
Wenshuai Zhu
中科院分区：
文献类型：
--
作者：
Shuhua Fan;Yongli Wang;Xian Wang;Li Huang;Yunxia Zhang;Xiaomeng Liu;Wenshuai Zhu
体外重折叠试验可用于研究表位肽与主要组织相容性复合体（MHC）I类分子结合的亲和力和稳定性，所述I类分子是肽呈递给细胞毒性T淋巴细胞（CTL）的关键因素。CTL对肽表位的识别对于预防甲型流感病毒（IAV）感染至关重要。猪SLA-3* hs 0202分子的肽结合基序先前已被报道，并且与最丰富的人MHC等位基因HLA-A*0201的结合基序部分重叠。本研究对猪源IAV A/Beijing/01/2009（H1N1）毒株的全部蛋白质序列进行了筛选，共筛选出73个符合猪SLA-3* hs 0202或HLA-A*0201共有模体的9肽表位。然后，选择了14个肽，并通过改进的体外复性试验测试它们与SLA-3* hs 0202的亲和力。我们的结果表明，10个表位可以耐受凝胶过滤，表明这些表位与SLA-3* hs 0202形成稳定或部分稳定的复合物。10个表位中有8个先前已被报道为HLA-A2限制性表位，这意味着猪和人MHC I特异性之间的交叉反应性。此外，改进的微系统复性方法可以应用于肽的筛选，因为复性效率几乎保持不变的阳性肽（HA-KMN 9）进行尺寸排阻色谱和资源Q阴离子交换色谱。本研究结果为开发基于表位的疫苗控制IAV提供了新的见解，并增加了我们对猪分子免疫学的理解。
In vitro refolding assays can be used to investigate the affinity and stability of the binding of epitope peptides to major histocompatibility complex (MHC) class I molecules, which are key factors in the presentation of peptides to cytotoxic T lymphocytes (CTLs). The recognition of peptide epitopes by CTLs is crucial for protection against influenza A virus (IAV) infection. The peptide-binding motif of the swine SLA-3*hs0202 molecule has been previously reported and partly overlaps with the binding motif of the most abundant human MHC allele, HLA-A*0201. In this study, we screened all the protein sequences of the swine-origin epidemic IAV strain A/Beijing/01/2009 (H1N1), and a total of 73 9-mer epitope peptides were predicted to fit the consensus motif of the swine SLA-3*hs0202 or HLA-A*0201 molecule. Then, 14 peptides were selected, and their affinities to SLA-3*hs0202 were tested by a modified in vitro refolding assay. Our results show that ten epitopes could tolerate gel filtration, indicating that these epitopes formed stable or partly stable complexes with SLA-3*hs0202. Eight out of the ten epitopes have been previously reported as HLA-A2-restricted epitopes, which implied cross-reactivity between swine and human MHC I specificities. Furthermore, the modified mini-system refolding method could be applied for the screening of peptides because the refolding efficiency remained almost unchanged with the positive peptide (HA-KMN9) subjected to size-exclusion chromatography and Resource Q anion-exchange chromatography. The results presented here provide new insight into the development of epitope-based vaccines to control IAV and increase our understanding of swine molecular immunology.
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影响因子：
6.7
作者：
Macdonald IK;Harkiolaki M;Hunt L;Connelley T;Carroll AV;MacHugh ND;Graham SP;Jones EY;Morrison WI;Flower DR;Ellis SA
通讯作者：
Ellis SA
影响因子：
5.4
作者：
Nianzhi Zhang;J. Qi;Sijia Feng;F. Gao;Jun Liu;X. Pan;Rong Chen;Qirun Li;Zhaosan Chen;
通讯作者：
Nianzhi Zhang;J. Qi;Sijia Feng;F. Gao;Jun Liu;X. Pan;Rong Chen;Qirun Li;Zhaosan Chen;
影响因子：
3.6
作者：
Fan, Shuhua;Wang, Yongli;Xia, Chun
通讯作者：
Xia, Chun
DOI：
10.1073/pnas.1013387107
发表时间：
2010-11-02
影响因子：
11.1
作者：
Wang, Taia T.;Tan, Gene S.;Palese, Peter
通讯作者：
Palese, Peter
影响因子：
--
作者：
Harndahl, Mikkel;Justesen, Sune;Buus, Soren
通讯作者：
Buus, Soren