The NEDD8 modification pathway in plants.
The NEDD8 modification pathway in plants.
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DOI：
10.3389/fpls.2014.00103
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发表时间：
2014
影响因子：
5.6
通讯作者：
Schwechheimer C
中科院分区：
文献类型：
--
作者：
Mergner J;Schwechheimer C
NEDD 8在植物和酵母中也称为与泛素相关（RUB），是进化上保守的76个氨基酸的蛋白质，与泛素高度相关。像泛素一样，NEDD 8可以与靶蛋白缀合和解缀合，但与泛素不同，NEDD 8尚未报道形成类似于在不同的细胞过程中起作用的不同聚合物泛素链的链。NEDD 8修饰最为人所知的是cullin-RING E3泛素连接酶的cullin亚基的翻译后修饰。在这种情况下，结构分析表明，neddylation诱导的cullin，使泛素化底物的相互作用E2共轭酶附近的构象变化。反过来，NEDD 8去缀合使cullin RING连接酶复合物不稳定，从而允许通过交换因子CAND 1交换底物识别亚基。在植物中，neddylation和deneddylation途径的组成部分被确定为基于生长素和光响应缺陷的突变体，这些突变体的表征有助于阐明neddylation途径。最近，动物和植物系统的证据表明，NEDD 8缀合也可以以多种方式调节非cullin底物的行为或命运。在此，在适用的情况下，在来自植物的特定信号传导途径的背景下，呈现了关于NEDD 8加工、缀合和解缀合的现有知识。
NEDD8, in plants and yeasts also known as RELATED TO UBIQUITIN (RUB), is an evolutionarily conserved 76 amino acid protein highly related to ubiquitin. Like ubiquitin, NEDD8 can be conjugated to and deconjugated from target proteins, but unlike ubiquitin, NEDD8 has not been reported to form chains similar to the different polymeric ubiquitin chains that have a role in a diverse set of cellular processes. NEDD8-modification is best known as a post-translational modification of the cullin subunits of cullin-RING E3 ubiquitin ligases. In this context, structural analyses have revealed that neddylation induces a conformation change of the cullin that brings the ubiquitylation substrates into proximity of the interacting E2 conjugating enzyme. In turn, NEDD8 deconjugation destabilizes the cullin RING ligase complex allowing for the exchange of substrate recognition subunits via the exchange factor CAND1. In plants, components of the neddylation and deneddylation pathway were identified based on mutants with defects in auxin and light responses and the characterization of these mutants has been instrumental for the elucidation of the neddylation pathway. More recently, there has been evidence from animal and plant systems that NEDD8 conjugation may also regulate the behavior or fate of non-cullin substrates in a number of ways. Here, the current knowledge on NEDD8 processing, conjugation and deconjugation is presented, where applicable, in the context of specific signaling pathways from plants.
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