Activation of poly(adenosine diphosphate ribose) polymerase with UV irradiated and UV endonuclease treated SV 40 minichromosome.
Activation of poly(adenosine diphosphate ribose) polymerase with UV irradiated and UV endonuclease treated SV 40 minichromosome.
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用紫外线照射和紫外线核酸内切酶处理的 SV 40 微型染色体激活聚（二磷酸腺苷核糖）聚合酶。
DOI：
10.1016/0006-291x(81)91898-2
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发表时间：
1981
影响因子：
3.1
通讯作者：
Berger,NA
中科院分区：
文献类型：
--
作者：
Cohen,JJ;Berger,NA
SV 40微型染色体被用作真核染色质的分子模型，以探测负责聚（ADPR）聚合酶的UV刺激的损伤的性质。用50和1000 J/m2之间的剂量的微型染色体的紫外线照射并没有增加他们的能力，刺激纯化的聚（ADPR）聚合酶的活性。而紫外线照射后再用M. luteus UV核酸内切酶，它们刺激聚（ADPR）聚合酶的能力显着增加。当将组蛋白H1加入到聚（ADPR）聚合酶测定中时，这种刺激被完全抑制。这些研究表明，在一个纯化的体外系统中，单独由紫外线照射引起的损伤是不足以刺激聚（ADPR）聚合酶活性。只有当DNA在UV损伤位点被UV核酸内切酶切割时，才有聚（ADPR）聚合酶的刺激。
SV 40 minichromosomes were used as a molecular model of eukaryotic chromatin to probe the nature of the lesion responsible for UV stimulation of poly (ADPR) polymerase. UV irradiation of the minichromosomes with doses between 50 and 1000 J/m 2 did not increase their ability to stimulate the activity of purified poly (ADPR) polymerase. In contrast, when the minichromosomes were UV irradiated and then treated with M. luteus UV endonuclease, there was a marked increase in their ability to stimulate poly (ADPR) polymerase. This stimulation was completely suppressed when histone Hl was added to the poly (ADPR) polymerase assay. These studies demonstrate in a purified in vitro system that damage caused by UV irradiation alone is not sufficient to stimulate poly (ADPR) polymerase activity. Only when DNA is nicked at the site of UV damage by UV endonuclease is there stimulation of poly (ADPR) polymerase.