THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENOMIC RNA OF THE TOBAMOVIRUS TOBACCO MILD GREEN MOSAIC-VIRUS
THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENOMIC RNA OF THE TOBAMOVIRUS TOBACCO MILD GREEN MOSAIC-VIRUS
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DOI：
10.1016/0042-6822(90)90520-2
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发表时间：
1990-08-01
期刊：
影响因子：
3.7
通讯作者：
GARCIAARENAL, F
中科院分区：
文献类型：
--
作者：
SOLIS, I;GARCIAARENAL, F
测定了烟草轻绿花叶病毒(Tm-GMV)基因组RNA全序列。该序列与烟草花叶病毒(TMV)基因组RNA的同源性为64.4%，与番茄花叶病毒(ToMV)的同源性为66.0%。其基因组结构与烟草花叶病毒和烟草花叶病毒相似。5‘端开放阅读框(ORF)编码一个126K的多肽和一个183K的通读产物，其中存在核苷酸结合基序和聚合酶序列基序。第三个ORF编码28.5k的蛋白，与TMV和ToMV运动蛋白同源。在另外四种烟草病毒和两种TOBRAB病毒中发现了一个保守的核心，这表明了TABAMO-病毒和TOBRAB病毒在细胞间移动的共同机制。第四个ORF编码17.5k的外壳蛋白。TMGMV与其卫星病毒STMV的RNA同源性仅限于其3‘端，结构比较表明该区域可能决定了卫星/辅助病毒相互作用的性质。
The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus (TM-GMV) was determined. It shows a 64.4% sequence homology with the genomic RNA of tobacco mosiac virus (TMV) and 66.0% with that of tomato mosaic virus (ToMV). Its genomic organization is similar to that of TMV and ToMV. The 5'' proximal open reading frame (ORF) encodes a 126K polypeptide and a 183K readthrough product in which nucleotide-binding and polymerase-sequence motifs are found. The third ORF encodes a 28.5K protein homologous to TMV and ToMV movement proteins. A conserved core is found with four other tobamoviruses and two tobraviruses suggesting a common mechanism of cell-to-cell movement for tobamo- and tobraviruses. The fourth ORF encodes the 17.5K coat protein. Homology between the RNAs of TMGMV and its satellite virus STMV is limited to their 3'' termini, and structural comparisons suggest that this region may determine the nature of the satellite/helper virus interaction.