Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus.
Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus.
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DOI：
10.1016/j.femsle.2005.08.027
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发表时间：
2005-11
影响因子：
2.1
通讯作者：
M. El‐Bendary;F. Priest;J. Charles;W. Mitchell
中科院分区：
文献类型：
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作者：
M. El‐Bendary;F. Priest;J. Charles;W. Mitchell
用含有目的基因克隆部分的自杀质粒与大肠杆菌接合，制备了球形芽孢杆菌2362的spo 0A和spoIIAC基因的插入突变。经电镜、SDS-PAGE和Western印迹分析，突变体的表型与枯草芽孢杆菌的对应部分相似，并且没有晶体蛋白。与亲本菌株相比，突变体对按蚊幼虫的毒性大大降低。我们的结论是晶体蛋白的合成在这种细菌是依赖于早期孢子形成基因的表达。
Insertional mutations in thespo0AandspoIIACgenes ofBacillus sphaericus2362 were prepared by conjugation withEscherichia coliusing a suicide plasmid containing cloned portions of the target genes. The mutants resembled theirBacillus subtiliscounterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS–PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes.