Immunodetection after complete destaining of coomassie blue-stained proteins on immobilon-PVDF.
Immunodetection after complete destaining of coomassie blue-stained proteins on immobilon-PVDF.
复制标题
考马斯蓝染色蛋白在固定聚偏氟乙烯 (immobilon-PVDF) 上完全脱色后进行免疫检测。
DOI：
10.1016/0003-2697(92)90213-q
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发表时间：
1992
影响因子：
2.9
通讯作者：
Hamilton,DW
中科院分区：
文献类型：
--
作者：
Pryor,JL;Xu,W;Hamilton,DW
描述了一种简化免疫可检测蛋白质相对于其他电泳法蛋白质的定位的技术。蛋白质转印到聚偏二氟乙烯(PVDF)膜上，考马斯亮蓝R-250染色显示，并拍摄蛋白质图案的照片。考马斯蓝染色的PVDF膜然后用25%的醋酸/50%的甲醇溶液完全脱色，这样就可以在同一膜上进行后续的免疫染色。这项技术使用普通的实验室试剂，快速，并已被证明适用于各种蛋白质，使用单抗和多克隆抗体以及各种转印。
A technique that simplifies the localization of an immunodetectable protein in relation to the other electrophoresed proteins is described. Proteins are transblotted onto a polyvinylidene difluoride (PVDF) membrane and visualized by staining with Coomassie brilliant blue R-250, and a photograph of the protein pattern is taken. The Coomassie blue-stained PVDF membrane is then completely destained using a 25% acetic acid/50% methanol solution that allows subsequent immunostaining on the same membrane. The technique uses common laboratory reagents, is rapid, and has been shown to be applicable for a variety of proteins using both monoclonal and polyclonal antibodies and a variety of transblots.