Berberine Prolongs Life Span and Stimulates Locomotor Activity of Drosophila melanogaster.
Berberine Prolongs Life Span and Stimulates Locomotor Activity of Drosophila melanogaster.
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DOI：
10.4236/ajps.2012.327123
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发表时间：
2012-07
期刊：
影响因子：
--
通讯作者：
Summergrad P
中科院分区：
文献类型：
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作者：
Navrotskaya VV;Oxenkrug G;Vorobyova LI;Summergrad P
具有由色氨酸（TRP）形成的犬尿氨酸（KYN）缺陷的黑腹果蝇突变体比野生型果蝇具有更长的寿命。施用TRP-KYN代谢抑制剂α-甲基-TRP和5-甲基-TRY延长了野生型果蝇的寿命。这两种抑制剂都不适用于人类。小檗碱是从小檗科植物小蘖中分离得到的一种异喹啉类生物碱，是中国和印度传统医药中广泛使用的草药。小檗碱是催化TRP转化为KYN的酶的强抑制剂。考虑到这个特殊的功能，我们研究了小檗碱对野生型果蝇寿命和健康寿命的影响。结果表明，黄连素能延长雌蝇的平均寿命、中位寿命和最高寿命。小檗碱对子代蛹的数量无影响，但可降低子代蛹的致死率。小檗碱增加运动活动（垂直攀爬）。研究结果表明，小檗碱延长果蝇的寿命并提高其健康寿命。黄连素可能成为预防和治疗衰老及与衰老相关的医学和精神疾病的候选药物。
Drosophila melanogaster mutants with deficient kynurenine (KYN) formation from tryptophan (TRP) have longer life span than wild type flies. Administration of alpha-methyl-TRP and 5-methyl-TRY, the inhibitors of TRP-KYN metabolism, prolonged life span in wild-type flies. Both inhibitors are not available for human use. Berberine, an isoquinoline alkaloid isolated from Berberis aristata, is known as the herb widely used in traditional Chinese and Indian medicine. Berberin is a strong inhibitor of the enzyme catalyzing TRP conversion into KYN. Considering this particular feature we investigated the effect of berberine on life- and health-span in wild-type Drosophila melanogaster. The results of our study showed that Berberine extended mean, median and maximum life span of female flies. Berberine did not affect the number of pupae of filial generation and decreased their lethality. Berberine increased locomotor activity (vertical climbing). The results of the study suggest that berberine prolongs life- and improves health-span of Drosophila melanogaster. Berberine might be a candidate drug for prevention and treatment of aging and aging-associated medical and psychiatric disorders.