Structural requirements for formyl homooligopeptide chemoattractants.
Structural requirements for formyl homooligopeptide chemoattractants.
复制标题
甲酰基同寡肽化学引诱剂的结构要求。
DOI：
10.1021/bi00299a018
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Becker,EL
中科院分区：
文献类型：
--
作者：
Toniolo,C;Bonora,GM;Showell,H;Freer,RJ;Becker,EL
化合物的制备是为了确定主链长度和侧链Y位置上硫原子的存在的综合影响。在细胞松弛素B存在的情况下，检测每种多肽诱导兔腹膜多形核白细胞分泌颗粒酶的能力。同时，利用红外吸收光谱和圆二色谱对其在固体和溶液中的构象进行了分析。我们研究了这些多肽在不同极性的溶剂中，即氯仿、2，2，2-三氟乙醇、L、3，3，3-六氟丙烷-2-醇以及有机-水混合介质。确定了形成反平行链/3缔合结构和折叠结构的趋势。
Compounds were prepared to determine the combined effects of the main-chain length and the presence of a sulfur atom in side-chain y-and-positions. Each peptide was tested for its ability to induce rabbit peritoneal polymorphonuclear leukocytes in the presence of cytochalasin B to secrete granule enzymes. In parallel, a conformational analysis was carried out in the solid state and in solution, using infrared absorption and circular dichroism. Weexamined these peptides in solvents of widely different polarities, ie, chloroform, 2, 2, 2-trifluoroethanol, l, l, l, 3, 3, 3-hexafluoropropan-2-ol, and mixed organic-aqueous media. The tendencies to form antiparallel-chain/3-associated and folded structures were determined.