Overexpression of Family with Sequence Similarity 83, Member A (FAM83A) Predicts Poor Clinical Outcomes in Lung Adenocarcinoma
Overexpression of Family with Sequence Similarity 83, Member A (FAM83A) Predicts Poor Clinical Outcomes in Lung Adenocarcinoma
复制标题
具有序列相似性的家族 83，成员 A (FAM83A) 的过度表达预示着肺腺癌的不良临床结果
DOI：
10.12659/msm.910804
复制
发表时间：
2019-06-08
影响因子：
3.1
通讯作者：
Yu, Ben-Tong
中科院分区：
文献类型：
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作者：
Zhang, Ling-Tao;Lin, Ye-Chun;Yu, Ben-Tong
本研究旨在探讨序列相似性家族83成员A（FAM 83 A）在肺腺癌（LUAD）中的表达水平及其临床预后价值。材料/方法基于TCGA和Oncomine数据库，采用生物信息学挖掘方法预测LUAD和正常肺组织中FAM 83 A mRNA的差异表达水平。采用免疫组化法检测83例LUAD患者及其配对正常肺组织中FAM 83 A蛋白的表达。采用卡方检验分析LUAD中FAM 83 A差异表达与临床病理因素的相关性。采用Kaplan-Meier单因素和考克斯多因素生存分析研究LUAD患者中FAM 83 A表达的临床预后价值。结果TCGA和Oncomine数据库结果显示，LUAD组织中FAM 83 A mRNA的表达水平明显高于正常肺组织（P<0.05）。免疫组化结果显示，FAM 83 A在LUAD中的高阳性率为73.49%（61/83），而在正常肺组织中的高阳性率仅为22.89%（19/83）。LUAD患者中FAM 83 A mRNA或高蛋白表达组的OS时间显著低于FAM 83 A mRNA或低蛋白表达组（P均<0.05）。考克斯多因素生存分析显示FAM 83 A差异表达水平（低vs.高）是预测LUAD患者预后的唯一独立因素（P=0.001）。结论FAM 83 A在LUAD中过表达，FAM 83 A过表达可作为LUAD患者预后不良的独立因素。
Background The aim of this study was to explore the expression levels of family with sequence similarity 83, member A (FAM83A) in lung adenocarcinoma (LUAD) and investigate its clinical prognostic value. Material/Methods Bioinformatics mining methods were used to predict the differential expression levels of FAM83A mRNA in LUAD and normal lung tissues based on the TCGA and Oncomine databases. Immunohistochemical staining was performed to demonstrate the FAM83A protein expression levels in 83 cases of LUAD combined with paired normal lung tissues. The correlation between clinicopathologic factors and FAM83A differential expression levels in LUAD was explored by the chi-square test. Kaplan-Meier univariate and Cox multivariate survival analyses were performed to investigate the clinical prognostic value of FAM83A expression in LUAD patients. Results Results from TCGA and Oncomine databases revealed that FAM83A mRNA expression level was significantly higher in LUAD than that in normal lung tissues (both P<0.05). Immunohistochemical findings demonstrated that the high positive rate of FAM83A in LUAD was 73.49% (61/83), while that of matched normal lung tissues was only 22.89% (19/83). Moreover, LUAD patients with FAM83A mRNA or high protein levels had dramatically lower OS times than those with FAM83A mRNA or low protein levels (All P<0.05). Lastly, Cox multivariate survival analysis showed that FAM83A differential expression level (low vs. high) was the only independent factor predicting the prognosis of LUAD patients (P=0.001). Conclusions FAM83A was overexpressed in LUAD, and FAM83A overexpression could be used as an independent factor of poor prognosis in LUAD patients.