One-megabase sequence analysis of the human immunoglobulin lambda gene locus
One-megabase sequence analysis of the human immunoglobulin lambda gene locus
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DOI：
10.1101/gr.7.3.250
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发表时间：
1997-03-01
期刊：
影响因子：
7
通讯作者：
Shimizu, N
中科院分区：
文献类型：
--
作者：
Kawasaki, K;Minoshima, S;Shimizu, N
已经确定了总共1，025，415个碱基的核苷酸序列，包括整个人免疫球蛋白λ基因座。这是有史以来最大的连续人类DNA序列。序列数据揭示了36个潜在活性V-λ基因片段、33个假基因片段和7个J（λ）-C-λ的组织。在这69个有功能或无功能的V-λ基因片段中，有32个是新发现的。这些V-λ基因片段位于五个基因丰富的簇内，并基于序列同一性分为五个家族。五个潜在的活性nonimmunoglobulin基因也被检测到在λ基因座，和其他两个基因中观察到的上游区域。序列组织表明，大的DNA重复多样化的V-lambda基因片段的种系库。
A total of 1,025,415 bases of nucleotide sequence, including the entire human immunoglobulin lambda gene locus has been determined. This is the largest contiguous human DNA sequence ever published. The sequence data revealed the organization of 36 potentially active V-lambda gene segments, 33 pseudogene segmnets, and seven J(lambda)-C-lambda. Among these 69 functional or nonfunctional V-lambda gene segments, 32 were newly discovered. These V-lambda gene segments are located within five gene-rich clusters and are divided into five clans based on sequence identity. Five potentially active nonimmunoglobulin genes were also detected within the lambda gene locus, and two other genes were observed in the upstream region. Sequence organization suggests that large DNA duplications diversified the germ-line repertoire of the V-lambda gene segments.